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PARP1 Antibody (702343) in ICC/IF
For immunofluorescence analysis, Staurosporine (1 uM, 16 h) treated HeLa cells were fixed and permeabilized for detection of endogenous PARP cleavage using Anti-Cleaved PARP beginning at N214 Recombinant Rabbit Monoclonal Antibody (Product # 702343, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Cleaved PARP protein (green), Panel b) is stained for nucl... View More
产品信息
702343
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
8H2L9
抗原
Peptide corresponding to Human PARP1 (aa 215-225)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2633085
产品详细信息
This antibody is predicted to react with Monkey, Pig and Rabbit
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); ADPRT 1; ARTD1; DNA ADP-ribosyltransferase PARP1; NAD(+) ADP-ribosyltransferase 1; NAD(+) pADPRT-1; PARP-1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; Poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; Protein poly-ADP-ribosyltransferase PARP1
基因别名: ADPRT; ADPRT 1; ADPRT1; ARTD1; pADPRT-1; PARP; PARP-1; PARP1; PPOL
UniProt ID: (Human) P09874
Entrez Gene ID: (Human) 142
DNA binding
DNA ligase (ATP) activity
NAD+ ADP-ribosyltransferase activity
protein binding
transcription factor binding
zinc ion binding
enzyme binding
protein kinase binding
identical protein binding
histone deacetylase binding
poly(A) RNA binding
protein N-terminus binding
NAD binding
R-SMAD binding
DNA metabolism protein
negative regulation of transcription from RNA polymerase II promoter
nucleotide-excision repair, DNA damage recognition
nucleotide-excision repair, DNA duplex unwinding
double-strand break repair via homologous recombination
lagging strand elongation
DNA repair
nucleotide-excision repair, preincision complex stabilization
nucleotide-excision repair, preincision complex assembly
nucleotide-excision repair, DNA incision, 3'-to lesion
nucleotide-excision repair, DNA incision, 5'-to lesion
double-strand break repair
transcription from RNA polymerase II promoter
protein ADP-ribosylation
cellular response to DNA damage stimulus
mitochondrion organization
transforming growth factor beta receptor signaling pathway
positive regulation of cardiac muscle hypertrophy
protein autoprocessing
protein sumoylation
signal transduction involved in regulation of gene expression
macrophage differentiation
mitochondrial DNA metabolic process
cellular response to insulin stimulus
nucleotide-excision repair, DNA incision
cellular response to oxidative stress
protein modification process
DNA damage response, detection of DNA damage
mitochondrial DNA repair
positive regulation of transcription from RNA polymerase II promoter
DNA ligation involved in DNA repair
positive regulation of SMAD protein import into nucleus
protein poly-ADP-ribosylation
global genome nucleotide-excision repair
regulation of cellular protein localization
negative regulation of telomere maintenance via telomere lengthening
ATP generation from poly-ADP-D-ribose
positive regulation of transcription regulatory region DNA binding
