Invitrogen

PASK Polyclonal Antibody

PASK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PASK Antibody (PA5-29309) in IHC (P)

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using PASK (Product # PA5-29309) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

PASK Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

PASK Antibody — Generation of conditional Pask alleles and deletion in beta or alpha cells. (a) Knockout (KO) strategy. Generation of beta (PaskBKO) and alpha (PaskAKO) cell-specific Pask KO mice by Cre-mediated excision of exons from 12 to 15 encoding the PASK Ser/Thr kinase domain. Human PASK cDNA (modified from [22]) with the Ser/Thr kinase domain encoding exons circled in purple and enlarged in the black box-a view from the back of PASK kinase domain (amino acids 977-1300) crystal structure (PBD code 3DLS) with the bound ADP molecule in evidence [43]. (b) Mouse Pask gene structure (modified from [22]) and the location of the loxP sites. (c) Example of a genotyping gel indicating the presence of WT and recombined (Cond) alleles. (d) Pask and Ins2 gene expression measured by qPCR in PaskBKO (BKO) and control (WT) mouse islets. Immunohistochemical analysis of pancreatic sections from PaskBKO (e) and PaskAKO (f) mice and controls, with PASK revealed using Alexa 568 (red), and insulin or glucagon revealed using Alexa 488 (green). Insets i-iv are magnified areas, as shown in the main images. Insets v-viii are example images of no-primary-antibody controls for each of the genotypes, with insets v and vii showing the DAPI staining for nuclei; scale bars, 100 µm. PASK fluorescence was displayed in 87.6 ± 1.5% of insulin-positive cells in WT islets vs 10.5 ± 2.3% in PaskBKO islets; n = 7 mice per genotype for both PaskBKO and PaskAKO mice; *p < 0.05 and **p < 0.01 by Student’s t test. Data were ob {KO}

产品信息

PA5-29309

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

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产品规格

种属反应

Human, Mouse

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 982 and 1227 of Human PASK

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 10% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2546785

产品详细信息

Recommended positive controls: mouse brain.

Predicted reactivity: Mouse (91%), Rat (90%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

PASK is an evolutionarily conserved protein present in yeast, flies, and mammals. PAS domains regulate the function of many intracellular signaling pathways in response to both extrinsic and intrinsic stimuli. It is a conserved gene product found in yeast as well as mammals. PASKIN contains two domains (PAS A and PAS B) as well as a serine/threonine kinase domain related to AMP kinases. PASKIN activity leads to decreased carbohydrate storage, as well as increased protein synthesis. PASKIN-dependent phosphorylation inhibits the activity of mammalian glycogen synthase.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Cell type-specific deletion in mice reveals roles for PAS kinase in insulin and glucagon production.

Diabetologia

Semplici F,Mondragon A,Macintyre B,Madeyski-Bengston K,Persson-Kry A,Barr S,Ramne A,Marley A,McGinty J,French P,Soedling H,Yokosuka R,Gaitan J,Lang J,Migrenne-Li S,Philippe E,Herrera PL,Magnan C,da Silva Xavier G,Rutter GA