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PAX5 Antibody (17-9918-80) in Flow
Intracellular staining of normal human peripheral blood cells with Anti-Human CD19 PE-Cy7 (Product # 25-0199-42) and 0.125 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or 0.125 µg of Anti-Human/Mouse Pax5 APC (right) using the Foxp3 Staining Buffer Set (Product # 00-5523-00). Cells in the lymphocyte gate were used for analysis.
产品信息
17-9918-80
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Human,
Mouse
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
1H9
偶联物
激发/发射光谱
651/660 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10734230
产品详细信息
Description: The monoclonal antibody 1H9 recognizes both mouse and human Pax5. Pax5, also known as BSAP (B cell specific activator protein), is a member of the paired box (pax) family of transcription factors. Pax5 is the only member of the pax family of transcription factors that is expressed in hematopoietic cells. During embryogenesis, Pax5 is transiently expressed in the brain of mice and in the mesencephalon and spinal cord of humans. Its expression is upregulated early in B cell development at the time of B cell commitment and is maintained throughout most subsequent stages. Suppression of Pax5 is essential for expression of Blimp-1 and the terminal differentiation of plasma cells. In the spleen, expression of Pax5 is higher in marginal zone B cells (B220+ CD21high CD23low) than in other B cells, especially the transition 1 stage (B220+ CD21- CD23-). In addition to its role in B cell development, Pax5 also affects VH-DJH heavy chain recombination as well as influencing the expression of many other B and non-B cell related proteins. Pax5 expression is correlated with many neoplasms. In diffuse large B cell lymphomas (DLBCL) and non-Hodgkin lymphomas Pax5 is often mutated while in B-cell ALL, expression levels are high. Additionally, translocation with elastin, IGH, ETV6, FOXP1, and EVI3 have been identified.
eBioscience recommends that the Foxp3 Buffers (Product # 00-5521) be used for optimal results when using this antibody for intracellular staining and flow cytometric analysis.
Applications Reported: This 1H9 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 1H9 antibody has been tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00). This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
PAX5 is a member of the paired box (PAX) family of transcription factors. The PAX proteins are important regulators in early development and alterations in the expression of their genes are thought to contribute to neoplastic transformation. PAX5 is the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages, of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX5 may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis. Mutations in the gene can result in leukemia, acute lymphoblastic 3.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: B-cell lineage specific activator; B-cell-specific transcription factor; BSAP; paired box gene 5; paired box homeotic gene 5; Paired box protein Pax-5; paired domain gene 5; Pax5 variant 1; Pax5 variant 2; transcription factor PAX 5
基因别名: ALL3; BSAP; EBB-1; KLP; Pax-5; PAX5
UniProt ID: (Human) Q02548, (Mouse) Q02650
Entrez Gene ID: (Human) 5079, (Mouse) 18507
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
multicellular organism development
spermatogenesis
nervous system development
aging
organ morphogenesis
lateral ventricle development
cerebral cortex development
cell differentiation
adult behavior
skeletal muscle cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
embryonic cranial skeleton morphogenesis
negative regulation of histone H3-K9 methylation
humoral immune response
