Invitrogen

PAX5 Monoclonal Antibody (6I4)

PAX5 Antibody in Immunocytochemistry (ICC/IF)

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PAX5 Antibody (MA5-36223) in ICC/IF

Immunocytochemistry analysis of PAX5 in U20S cell. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in PAX5 monoclonal antibody (Product # MA5-36223) at a dilution of 2 µg/mL (overnight, 4°C), followed by DyLight 488 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 1:100.

PAX5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PAX5 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of PAX5 was observed in Raji, Ramos and Daudi but not in Jurkat, A549, PANC-1 and K-562 which are reported to be negative using Anti-PAX5 Monoclonal Antibody (6I4) (Product # MA5-36223) in western blot. {RE}

产品信息

MA5-36223

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

1-5 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

6I4

抗原

E. coli-derived human PAX5 recombinant protein (Position: R217-A282).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2884057

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

PAX5 is a member of the paired box (PAX) family of transcription factors. The PAX proteins are important regulators in early development and alterations in the expression of their genes are thought to contribute to neoplastic transformation. PAX5 is the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages, of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX5 may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis. Mutations in the gene can result in leukemia, acute lymphoblastic 3.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: B-cell lineage specific activator; B-cell-specific transcription factor; BSAP; paired box gene 5; paired box homeotic gene 5; Paired box protein Pax-5; paired domain gene 5; Pax5 variant 1; Pax5 variant 2; transcription factor PAX 5

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基因别名: ALL3; BSAP; EBB-1; KLP; Pax-5; PAX5; RGD1565236

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UniProt ID: (Human) Q02548, (Mouse) Q02650

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Entrez Gene ID: (Human) 5079, (Rat) 500453, (Mouse) 18507

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功能

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding protein binding DNA binding transcription factor activity, sequence-specific DNA binding

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