Invitrogen

PAX8 Monoclonal Antibody (1F8-3A8)

PAX8 Antibody in Immunocytochemistry (ICC/IF)

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PAX8 Antibody (MA1-117) in ICC/IF

Immunofluorescent analysis of PAX8 (green) in 293T (left panel) and COS7 cells (right panel). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a PAX8 monoclonal antibody (Product # MA1-117) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502) a... View More

PAX8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PAX8 Antibody in Immunohistochemistry (IHC)

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PAX8 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. COS-7 cells were transfected with PAX8 siRNA and reduction of signal was observed in Western Blot using PAX8 Monoclonal Antibody (Product # MA1-117). {KD}

产品信息

MA1-117

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:20-1:200

免疫组化（冰冻） (IHC (F))

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免疫细胞化学 (ICC/IF)

1:50-1:200

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流式细胞分析 (Flow)

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产品规格

种属反应

Human, Mouse, Non-human primate

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

1F8-3A8

抗原

Purified internal fragment of human recombinant PAX8 expressed in E. coli.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with 1mg/mL BSA, 30% glycerol

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2536828

产品详细信息

Western blot analysis of MA1-117 detects a prominent ~48 kDa protein in human adenocarcinoma SKOV-3 and SV40 transformed African green monkey (Cercopithecus aethiops) kidney cells. In SKOV-3 cells, additional unknown low molecular bands are also detected. Specificity of the antibody was confirmed in HeLa (negative control) and 293T cells overexpressing full length PAX8.

靶标信息

This gene encodes a member of the paired box family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The Repertoire of Serous Ovarian Cancer Non-genetic Heterogeneity Revealed by Single-Cell Sequencing of Normal Fallopian Tube Epithelial Cells.

Cancer cell

Hu Z,Artibani M,Alsaadi A,Wietek N,Morotti M,Shi T,Zhong Z,Santana Gonzalez L,El-Sahhar S,Carrami EM,Mallett G,Feng Y,Masuda K,Zheng Y,Chong K,Damato S,Dhar S,Campo L,Garruto Campanile R,Soleymani Majd H,Rai V,Maldonado-Perez D,Jones S,Cerundolo V,Sauka-Spengler T,Yau C,Ahmed AA

MA1-117 was used in Immunohistochemistry (Frozen) to lay the foundation for accurate prognostic and therapeutic stratification of SOC.

Mon Feb 10 00:00:00 UTC 2020

Derivation and 97% Purification of Human Thyroid Cells From Dermal Fibroblasts.

Frontiers in endocrinology

Ma R,Shi R,Morshed SA,Latif R,Davies TF

MA1-117 was used in Immunocytochemistry, Flow Cytometry to demonstrate that mature adult dermal fibroblasts can be matured into human iPSCs which have the potential to form functional thyroid follicular cells.

Thu Jun 03 00:00:00 UTC 2021

Derivation and 97% Purification of Human Thyroid Cells From Dermal Fibroblasts.

Frontiers in endocrinology

Ma R,Shi R,Morshed SA,Latif R,Davies TF

Thu Jun 03 00:00:00 UTC 2021

Derivation and 97% Purification of Human Thyroid Cells From Dermal Fibroblasts.

Frontiers in endocrinology

Ma R,Shi R,Morshed SA,Latif R,Davies TF

Thu Jun 03 00:00:00 UTC 2021

Derivation and 97% Purification of Human Thyroid Cells From Dermal Fibroblasts.

Frontiers in endocrinology

Ma R,Shi R,Morshed SA,Latif R,Davies TF

Thu Jun 03 00:00:00 UTC 2021

A Stem Cell Surge During Thyroid Regeneration.

Frontiers in endocrinology

Ma R,Morshed SA,Latif R,Davies TF

MA1-117 was used in Immunohistochemistry, Flow Cytometry to explore the mechanism behind thyroid stem cell and progenitor cells in the regeneration and repair process after injury.

Tue May 25 00:00:00 UTC 2021

A Stem Cell Surge During Thyroid Regeneration.

Frontiers in endocrinology

Ma R,Morshed SA,Latif R,Davies TF

MA1-117 was used in Immunohistochemistry, Flow Cytometry to explore the mechanism behind thyroid stem cell and progenitor cells in the regeneration and repair process after injury.

Tue May 25 00:00:00 UTC 2021

A Stem Cell Surge During Thyroid Regeneration.

Frontiers in endocrinology

Ma R,Morshed SA,Latif R,Davies TF

MA1-117 was used in Immunohistochemistry, Flow Cytometry to explore the mechanism behind thyroid stem cell and progenitor cells in the regeneration and repair process after injury.

Tue May 25 00:00:00 UTC 2021

Acute renal failure due to small cell neuroendocrine carcinoma of the left kidney: A case report.

Oncology letters

Haberal HB,Tonyali Ş,Baydar DE,Bilen CY

MA1-117 was used in Immunohistochemistry to assess a female patient with small cell carcinoma of the left kidney.

Wed Nov 01 00:00:00 UTC 2017

Hypoxia promotes thyroid differentiation of native murine induced pluripotent stem cells.

The International journal of developmental biology

Yang Y,Lu Y,Chen T,Zhang S,Chu B,Gong Y,Zhao W,Zhu J,Liu Y

MA1-117 was used in Immunocytochemistry-immunofluorescence to find that hypoxia promoted adhesion and outgrowth of embryoid bodies (EBs) derived from murine iPS cells.