Invitrogen

PBRM1 Recombinant Rabbit Monoclonal Antibody (ARC1820)

查看其他16个PBRM1抗体

抗体检测数据 (4)

PBRM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PBRM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PBRM1 Antibody (MA5-37814) in IHC (P)

Immunohistochemistry analysis of PBRM1 in paraffin-embedded human appendix. Samples were incubated with PBRM1 Monoclonal antibody (Product # MA5-37814) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

PBRM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PBRM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PBRM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PBRM1 Antibody in Western Blot (WB)

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PBRM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PBRM1 in paraffin-embedded human appendix. Samples were incubated with PBRM1 Monoclonal antibody (Product # MA5-37814) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

PBRM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PBRM1 in paraffin-embedded mouse kidney. Samples were incubated with PBRM1 Monoclonal antibody (Product # MA5-37814) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

PBRM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PBRM1 in paraffin-embedded rat ovary. Samples were incubated with PBRM1 Monoclonal antibody (Product # MA5-37814) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

PBRM1 Antibody in Western Blot (WB) — Western blot analysis of PBRM1 in extracts of various cell lines. Samples were incubated with PBRM1 Monoclonal antibody (Product # MA5-37814) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3 min.

产品信息

MA5-37814

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:200-1:800

-

酶联免疫吸附实验 (ELISA)

1 µg/mL

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

ARC1820

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 530-680 of human PBRM1 (Q86U86)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2897738

产品详细信息

Positive Samples: HeLa, NIH/3T3, Jurkat, Rat brain

Immunogen sequence: VLEAREPGSG RRLCDLFMVK PSKKDYPDYY KIILEPMDLK IIEHNIRNDK YAGEEGMIED MKLMFRNARH YNEEGSQVYN DAHILEKLLK EKRKELGPLP DDDDMASPKL KLSRKSGISP KKSKYMTPMQ QKLNEVYEAV KNYTDKRGRR L

靶标信息

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 2310032M22Rik; BAF180; BRG1-associated factor 180; hPB1; polybromo 1; Polybromo-1D; Protein polybromo-1

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基因别名: 2610016F04Rik; AI507524; BAF180; PB1; PBRM1; RGD1565549

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UniProt ID: (Human) Q86U86

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Entrez Gene ID: (Human) 55193, (Rat) 306254, (Mouse) 66923

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DNA binding chromatin binding protein binding molecular_function chromatin/chromatin-binding, or -regulatory protein

chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated mitotic nuclear division negative regulation of cell proliferation covalent chromatin modification placenta development heart development biological_process blood vessel remodeling heart morphogenesis epicardium-derived cardiac endothelial cell differentiation chromatin modification positive regulation of transcription, DNA-templated cardiac vascular smooth muscle cell development vasculogenesis involved in coronary vascular morphogenesis