Bethyl Laboratories

PBRM1 Recombinant Rabbit Monoclonal Antibody (BL-39-2C3)

查看其他16个PBRM1抗体

抗体检测数据 (8)

PBRM1 Antibody in Immunocytochemistry (ICC/IF)

PBRM1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 8

PBRM1 Antibody (A700-019) in ICC/IF

Detection of human PBRM1 in FFPE MCF7 cells by immunocytochemistry. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (Product # A700-019) (lot 4). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

PBRM1 Antibody in Immunocytochemistry (ICC/IF)

PBRM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PBRM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PBRM1 Antibody in Western Blot (WB)

PBRM1 Antibody in Western Blot (WB)

PBRM1 Antibody in Western Blot (WB)

PBRM1 Antibody in Flow Cytometry (Flow)

PBRM1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

PBRM1 Antibody in Immunocytochemistry (ICC/IF) — Detection of human PBRM1 in FFPE MCF7 cells by immunocytochemistry. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (Product # A700-019) (lot 4). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

PBRM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Detection of human PBRM1 in FFPE breast by immunohistochemistry-IF. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (Product # A700-019) (Lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: Opal™. Counterstain: DAPI.

PBRM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Detection of human PBRM1 in FFPE breast carcinoma by immunohistochemistry. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (Product # A700-019) (lot 4). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

PBRM1 Antibody in Western Blot (WB) — Detection of mouse PBRM1 by western blot. Samples: Whole cell lysate (50 µg) from BW5147.3, EL4, mIMCD-3, RenCa, NIH 3T3, TCMK-1, C2C12, A20, Ba/F3, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (Product # A700-019 lot 4) used at 1:1,000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody (A304-523A).

PBRM1 Antibody in Western Blot (WB) — Detection of human PBRM1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (Product # A700-019 lot 4) used at 1:1,000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody (A304-523A).

PBRM1 Antibody in Western Blot (WB) — Detection of mouse PBRM1 by Simple Western™. Samples: Whole cell lysate (0.08 mg/mL) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (Product # A700-019) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.

PBRM1 Antibody in Flow Cytometry (Flow) — Detection of human PBRM1 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-PBRM1 recombinant monoclonal (BL-39-2C3) (Product # A700-019) (lot 4) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG (A120-101D2).

PBRM1 Antibody in Immunoprecipitation (IP) — Detection of human PBRM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (Product # A700-019) (lot 3). For blotting immunoprecipitated PBRM1, A700-019 was used at 1:1,000. Detection: Chemiluminescence with an exposure time of 10 seconds.

产品信息

A700-019

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

-

免疫组化（石蜡） (IHC (P))

1:100-1:500

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

流式细胞分析 (Flow)

0.8 µL per 1 x 10^6 cells

-

免疫沉淀 (IP)

5 µl/mg lysate

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

BL-39-2C3

抗原

Recombinant protein representing residues 1-293 of human Polybromo 1.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

purified

保存液

BBS, pH 8.2, with 0.1% BSA

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Wet ice

产品详细信息

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For ICC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

靶标信息

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: 2310032M22Rik; BAF180; BRG1-associated factor 180; hPB1; Polybromo-1D; Protein polybromo-1

查看更多收起

基因别名: 2610016F04Rik; AI507524; BAF180; PB1; PBRM1

查看更多收起

UniProt ID: (Human) Q86U86

查看更多收起

Entrez Gene ID: (Human) 55193, (Mouse) 66923

查看更多收起

DNA binding chromatin binding protein binding chromatin/chromatin-binding, or -regulatory protein

chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated mitotic nuclear division negative regulation of cell proliferation covalent chromatin modification placenta development blood vessel remodeling heart morphogenesis epicardium-derived cardiac endothelial cell differentiation heart development chromatin modification positive regulation of transcription, DNA-templated cardiac vascular smooth muscle cell development vasculogenesis involved in coronary vascular morphogenesis