Invitrogen

PBX3 Polyclonal Antibody

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抗体检测数据 (2)

PBX3 Antibody in Immunocytochemistry (ICC/IF)

PBX3 Antibody in Immunocytochemistry (ICC/IF)

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PBX3 Antibody (PA5-102989) in ICC/IF

Immunocytochemistry analysis of PBX3 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with PBX3 polyclonal antibody (Product # PA5-102989) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

PBX3 Antibody in Immunocytochemistry (ICC/IF)

PBX3 Antibody in Western Blot (WB)

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PBX3 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of PBX3 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with PBX3 polyclonal antibody (Product # PA5-102989) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

PBX3 Antibody in Western Blot (WB) — Western blot analysis of PBX3 in K562 cell lysate. Samples were incubated with PBX3 polyclonal antibody (Product # PA5-102989).

产品信息

PA5-102989

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human PBX3(Accession P40426), corresponding to amino acid residues Q9-Q59.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2852362

产品详细信息

Antibody detects endogenous levels of total PBX3.

靶标信息

PBX3 is a transcriptional activator that binds the sequence 5'-ATCAATCAA-3'.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Homeobox protein PBX3; pre-B-cell leukemia homeobox 3; Pre-B-cell leukemia transcription factor 3

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基因别名: PBX3

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UniProt ID: (Human) P40426, (Mouse) O35317

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Entrez Gene ID: (Human) 5090, (Mouse) 18516

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RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding sequence-specific DNA binding homeodomain transcription factor

regulation of respiratory gaseous exchange by neurological system process transcription from RNA polymerase II promoter anterior compartment pattern formation posterior compartment specification respiratory gaseous exchange adult locomotory behavior dorsal spinal cord development positive regulation of transcription from RNA polymerase II promoter neuron development transcription, DNA-templated regulation of transcription, DNA-templated