Invitrogen

PCAF Recombinant Rabbit Monoclonal Antibody (13H24L4)

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PCAF Antibody (703379) in WB

Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of LNCaP (Lane 1), PC-3 (Lane 2), HeLa (Lane 3), HCT 116 (Lane 4), U-2 OS (Lane 5) and C2C12 (Lane 6). The blot was probed with Anti-PCAF Recombinant Rabbit Monoclonal Antibody (Product # 703379, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution). A ~93 kDa band corresponding to PCAF was observed across the cell lines tested.

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PCAF Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. LNCaP cells were transfected with PCAF siRNA and a decrease in signal intensity was observed in Western blot application using Anti-PCAF Recombinant Rabbit Monoclonal Antibody (Product # 703379). {KD}

产品信息

703379

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

13H24L4

抗原

Peptides corresponding to human PCAF [(aa416-aa435) (aa818-aa832)]

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2784580

产品详细信息

This antibody is predicted to react with Monkey, Cat, Pig.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

PCAF (histone acetyltransferase KAT2B) functions as a histone acetyltransferase (HAT) to promote transcriptional activation. It has significant histone acetyltransferase activity with core histones (H3 and H4) and nucleosome core particles. PCAF also acetylates non-histone proteins such as ACLY, PLK4, and TBX5. It inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. PCAF also acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers. It is involved in heart and limb development by mediating acetylation of TBX5 and acetylation regulating nucleocytoplasmic shutting of TBX5.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CREBBP-associated factor; Histone acetylase PCAF; Histone acetyltransferase KAT2B; Histone acetyltransferase PCAF; K(lysine) acetyltransferase 2B; Lysine acetyltransferase 2B; P/ PCAF; P/CAF; P300/CBP-associated factor; Spermidine acetyltransferase KAT2B

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基因别名: A930006P13Rik; AI461839; AW536563; CAF; KAT2B; P/CAF; PCAF

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UniProt ID: (Human) Q92831, (Mouse) Q9JHD1

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Entrez Gene ID: (Human) 8850, (Mouse) 18519

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