Invitrogen

PCK1 Polyclonal Antibody

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PCK1 Antibody (720266) in WB

Western blot was performed using Anti-PCK1 Polyclonal Antibody (Product # 720266) and a 34 kDa band corresponding to PCK1 was observed across the panel tested. Whole cell extracts (30 µg lysate) of MCF-7 (Lane 1), MDA-MB-231 (Lane 2), Hep G2 (Lane 3), HEK-293 (Lane 4), Caco-2 (Lane 5), HCT 116 (Lane 6) and Mouse Liver (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product... View More

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产品信息

720266

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Protein corresponding to Human PCK1 (aa 472-620)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 30% glycerol, 0.1% BSA

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2633227

产品详细信息

These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.

This antibody is predicted to react with Monkey, Mouse, Sheep and Bovine

靶标信息

Phosphoenolpyruvate Carboxykinase 1 (PCK1) is a main control point for the regulation of gluconeogenesis. During gluconeogenesis PCK1 acts as the rate-limiting enzyme. PCK1 regulates the formation and maintenance of memory CH8(+) T-cells via gluconeogenesis. PCK1 aids in controlling the levels of metabolic intermediates in the citric acid cycle by regulating cataplerosis and anaplerosis. When glucose levels are high, PCK1 catalyzes the cataplerotic conversion of oxaloacetate to phosphoenolpyruvate (PEP). When they are low it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate. When phosphorylated at Ser-90 by AKT1, PCK1 acts as a protein kinase by reducing the binding affinity to oxaloacetate and promotes an atypical serine protein kinase activity using GTP as the donor. This activity regulates lipogenesis by disrupting the interaction between INSIG proteins and SCAP and promoting nuclear translocation of SREBP proteins and transcription of lipogenesis-related genes. An important paralog of this gene is PCK2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: HGNC:8724; MGC22652; PE PEPCKC; PEP carboxykinase; PEPCK-C; phosphoenolpyruvate carboxykinase (GTP); phosphoenolpyruvate carboxykinase 1 (soluble); phosphoenolpyruvate carboxykinase 1, cytosolic; phosphoenolpyruvate carboxykinase, cytosolic; phosphoenolpyruvate carboxykinase, cytosolic (GTP); Phosphoenolpyruvate carboxykinase, cytosolic [GTP]; phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase; Serine-protein kinase PCK1

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基因别名: AI265463; GTP; PCK; Pck-1; PCK1; PEPCK; PEPCK-C; PEPCK1; PEPCKC; RATPEPCK

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UniProt ID: (Human) P35558, (Rat) P07379, (Mouse) Q9Z2V4

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Entrez Gene ID: (Human) 5105, (Rat) 362282, (Mouse) 18534

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