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PCLO Antibody (MA5-45259) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Piccolo monoclonal antibody (Product # MA5-45259) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Endoplasmic Reticulum, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Ph... View More
产品信息
MA5-45259
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
6H9-B6
抗原
Full length recombinant protein (GenBank ID: BC001304.1)
偶联物
激发/发射光谱
482/675 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2931713
产品详细信息
1 µg/mL of MA5-45259 was sufficient for detection of Piccolo in 20 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat IgG:HRP as the secondary antibody.|Detects approximately 550kDa. No cross-reactivity to Bassoon.
靶标信息
The protein encoded by the PCLO gene is part of the presynaptic cytoskeletal matrix, which is involved in establishing active synaptic zones and in synaptic vesicle trafficking. Variations in this gene have been associated with bipolar disorder and major depressive disorder. Two transcript variants encoding different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Aczonin; Brain-derived HLMN protein; Multidomain presynaptic cytomatrix protein; multidomain presynaptic cytomatrix protein Piccolo; PCLO; presynaptic cytomatrix protein; Protein piccolo
基因别名: ACZ; aczonin; KIAA0559; PCH3; PCLO; Pico
UniProt ID: (Human) Q9Y6V0, (Mouse) Q9QYX7, (Rat) Q9JKS6
Entrez Gene ID: (Human) 27445, (Mouse) 26875, (Rat) 56768
