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PCP4 Antibody (PA5-103665) in ICC/IF
Immunocytochemistry analysis of PCP4 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with PCP4 polyclonal antibody (Product # PA5-103665) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-103665
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human PCP4(Accession P48539), corresponding to amino acid residues M1-A42.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2852999
产品详细信息
Antibody detects endogenous levels of total PCP4.
靶标信息
PCP-4 (Purkinje cell protein 4), also known as PCP4, is a calmodulin (CaM) regulatory protein that is highly expressed in neuronal cells. Through its IQ motif, PCP-4 mediates both the calcium-dependent binding properties of CaM and the rates of association and dissociation of calcium from the C-terminal domain of CaM. The IQ motif contains a serine residue which can be phosphorylated by all isoforms of protein kinase C (PKC). PCP-4 is implicated in uterine leiomyomas, as well as in neurodegenerative disorders such as Alzheimer's disease and Huntington's disease. Additionally, overexpression of PCP-4 is thought to play a role in cerebellar hypoplasia, a key feature of Down syndrome.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: brain specific polypeptide PEP19; Brain-specific antigen PCP-4; Brain-specific polypeptide PEP-19; Calmodulin regulator protein PCP4; Purkinje cell protein 4
基因别名: P16Rimb19; Pcp-4; PCP4; PEP-19; PEP19; PEPZ19
UniProt ID: (Human) P48539, (Mouse) P63054, (Rat) P63055
Entrez Gene ID: (Human) 5121, (Mouse) 18546, (Rat) 25510
osteoblast differentiation
negative regulation of protein kinase activity
response to osmotic stress
central nervous system development
positive regulation of neuron projection development
positive regulation of dopamine secretion
negative regulation of neuron apoptotic process
cellular response to monosodium glutamate
