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PCSK2 Antibody (PA1-058) in ICC/IF
Immunofluorescent analysis of PC2 (green) showing staining in the cytoplasm of HEK293 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a PC2 polyclonal antibody (Product # PA1-058) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (re... View More
产品信息
PA1-058
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Mouse,
Pig,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues E(622) L D E A V E R S L Q S I L R K N(638) of mouse PC2.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2158593
产品详细信息
PA1-058 detects prohormone convertase 2 (PC2) from human and mouse alpha pancreas cells.
PA1-058 has been successfully used in ICC/IF and Western blot procedures. By Western blot, this antibody detects an ~65 kDa protein representing PC2 from alpha TC 6 cell extract. This antibody is not recommended for Neuro-2a cells in immunfluorescent applications.
The PA1-058 immunogen is a synthetic peptide corresponding to residues E(622) L D E A V E R S L Q S I L R K N(638) of mouse PC2. This sequence is completely conserved between mouse and rat protein. PA1-058 synthetic peptide (PEP-138) is available for use in neutralization and control experiments.
靶标信息
The subtilisin-like prohormone convertase (PC) family is a group of cellular enzymes that cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. PC family members share structural similarities, which include a heterogeneous approximately 10 kDa amino-terminal proregion, a highly conserved approximately 55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. The subcellular localization of PC family members varies. Immunolocalization studies show that PC1 is found in the perinuclear region as well as the trans-Golgi network, whereas PC2 can be found in the trans-Golgi network as well as diffusely distributed in the peripheral cytoplasm.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: EC 3.4.21.94; KEX2-like endoprotease 2; NEC 2; Neuroendocrine convertase 2; PC2; Prohormone convertase 2; Proprotein convertase 2
基因别名: 6330411F23Rik; AI839700; NEC 2; NEC-2; NEC2; PC2; PCSK2; Phpp-2; SPC2
UniProt ID: (Human) P16519, (Mouse) P21661
Entrez Gene ID: (Human) 5126, (Mouse) 18549
