Invitrogen

PD-1 Monoclonal Antibody (7A11B1)

PD-1 Antibody in Immunohistochemistry (IHC)

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PD-1 Antibody (MA5-15780) in IHC

Immunofluorescence of PD-1 in human spleen tissue with PD-1 Monoclonal Antibody (7A11B1) (Product # MA5-15780) at 20 µg/mL. Green: PD-1 Antibody [7A11B1] (PM-5177) Blue: DAPI staining

PD-1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

PD-1 Antibody — Fig. 3 Effects of PD-1/PD-L1 overexpression and knockdown on brain cell death and neuronal degeneration after ICH. a ICH rats accepted intracerebroventricular injection of pDNA or siRNAs as indicated. b Western blot analysis of the efficiency of PD-1 and PD-L1 overexpression or knockdown in brain of ICH rats. Quantification of relative protein levels of PD-1 and PD-L1 was shown in c. c Data are expressed as mean +- SEM, n = 6. Triple asterisks indicate p < 0.001 vs. ICH + vector group; triple pound signs indicate p < 0.001 vs. ICH + control siRNA group. d Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. Sections were labeled by TUNEL ( green ) to detect apoptotic brain cells and counterstained with DAPI ( blue ) to detect nuclei. Arrows point to TUNEL-positive cells. Scale bar = 64 mum. e Percentage of TUNEL-positive cells. Data are expressed as mean +- SEM, n = 6. Triple asterisks indicate p < 0.001 vs. sham group; double pound signs indicate p < 0.01 vs. ICH + vector group; ampersand indicates p < 0.05, double ampersands indicate p < 0.01 vs. ICH + control siRNA group. f Fluoro-jade B (FJB) staining. Arrows point to FJB-positive cells. Scale bar = 64 mum. The number of FJB-positive brain cells was calculated. g Data are expressed as mean +- SEM, n = 6. Triple asterisks indicate p < 0.001 vs. sham group; double pound signs indicate p < 0.01 vs. ICH + vector group; double ampersands indicate p < 0.01, triple ampersands indicate p < 0.001 vs. ICH {KD}

产品信息

MA5-15780

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

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免疫组化 (IHC)

2.5 µg/mL

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免疫沉淀 (IP)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

7A11B1

抗原

A ~150 amino acid recombinant protein from near the amino terminus of mouse PD-1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_11152225

产品详细信息

A suggested positive control is PD-1 recombinant protein.

靶标信息

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Knockdown of Leptin Receptor Affects Macrophage Phenotype in the Tumor Microenvironment Inhibiting Breast Cancer Growth and Progression.

Cancers

Gelsomino L,Naimo GD,Malivindi R,Augimeri G,Panza S,Giordano C,Barone I,Bonofiglio D,Mauro L,Catalano S,Andò S

MA5-15780 was used in Immunohistochemistry to evaluate how the tumour cell-macrophage interaction and breast cancer phenotype are affected by leptin receptor knockdown.

Mon Jul 27 00:00:00 UTC 2020

Knockdown of Leptin Receptor Affects Macrophage Phenotype in the Tumor Microenvironment Inhibiting Breast Cancer Growth and Progression.

Cancers

Gelsomino L,Naimo GD,Malivindi R,Augimeri G,Panza S,Giordano C,Barone I,Bonofiglio D,Mauro L,Catalano S,Andò S

MA5-15780 was used in Immunohistochemistry to evaluate how the tumour cell-macrophage interaction and breast cancer phenotype are affected by leptin receptor knockdown.

Mon Jul 27 00:00:00 UTC 2020

Roles of programmed death protein 1/programmed death-ligand 1 in secondary brain injury after intracerebral hemorrhage in rats: selective modulation of microglia polarization to anti-inflammatory phenotype.

Journal of neuroinflammation

Wu J,Sun L,Li H,Shen H,Zhai W,Yu Z,Chen G

MA515780 was used in immunocytochemistry, immunohistochemistry - paraffin section, immunoprecipitation, and western blot to assess the critical role of programmed death protein 1/programmed death-ligand 1 in the polarization of microglia during intracerebral hemorrhage-induced secondary brain injury

Tue Feb 14 00:00:00 UTC 2017

Journal of neuroinflammation

Wu J,Sun L,Li H,Shen H,Zhai W,Yu Z,Chen G

Tue Feb 14 00:00:00 UTC 2017

Journal of neuroinflammation

Wu J,Sun L,Li H,Shen H,Zhai W,Yu Z,Chen G

Tue Feb 14 00:00:00 UTC 2017

生物信息学

蛋白别名: CD279; hPD1; mPD-1; programmed cell death 1 protein; Programmed cell death protein 1; programmed cell death protein 1-like; Protein PD-1; Protein PD1; sCD279; soluble CD279; systemic lupus erythematosus susceptibility 2

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基因别名: CD279; hPD-1; hPD-l; hSLE1; Ly101; PD-1; PD1; Pdc1; PDCD1; SLEB2

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UniProt ID: (Human) Q15116, (Mouse) Q02242

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Entrez Gene ID: (Human) 5133, (Rat) 301626, (Mouse) 18566

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功能

signal transducer activity protein binding molecular_function

参与通路