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PDE6D Antibody (PA1-726) in ICC/IF
Immunofluorescent analysis of PDE6 delta (green) showing staining in the cytoplasm and nucleus of B-3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a PDE6 delta polyclonal antibody (Product # PA1-726) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated wi... View More
产品信息
PA1-726
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Human,
Sheep
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic Peptides: M(1) S A K D E R A R (D/E) I L R G F K(16) C
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2283611
产品详细信息
PA1-726 detects PrBP/delta from bovine, human and sheep samples.
PA1-726 has been successfully used in Western blot, ICC/IF and immunohistochemistry procedures. By Western blot, this antibody detects a 17 kDa protein representing PrBP/delta from bovine and sheep retinal/optic nerve extracts as well as HeLa cell lysate. Immunohistochemical staining of PrBP/delta in bovine retinal cross-sections using PA1-726 results in staining of retinal outer segments.
PA1-726 immunizing peptides correspond to amino acid residues 1-16 from mouse and human PrBP/delta. This peptide
(Cat. # PEP-203) is available for use in neutralization and control experiments.
靶标信息
PrBP/delta, previously known as PDE6/delta, is the effector enzyme in the G protein-mediated signal transduction cascade in the visual system. There are five different subunits consisting of rod and cone specific catalytic subunits: alpha (Cone), alpha (Rod), and beta (Rod), the inhibitory subunit gamma, and subunit delta of unknown function (which likely interacts with many other proteins besides the PDE6 family). PRBP/delta has been implicated in the transport and membrane targeting of prenylated proteins (including PDE6) from their site of synthesis in the inner segment to their final destination in the outer segment of rods and cones (Norton et. al., 2005).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: GMP-PDE delta; phosphodiesterase 6D, cGMP-specific, rod, delta; Protein p17; Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta
基因别名: BOS_2158; JBTS22; PDE6D; PDED
UniProt ID: (Human) O43924, (Bovine) Q95142
Entrez Gene ID: (Human) 5147, (Bovine) 281976, (Sheep) 101108369
