Invitrogen

PDI Polyclonal Antibody

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PDI Antibody (PA5-77821) in WB

Western blot was performed using Anti-PDI Polyclonal Antibody (Product # PA5-77821) and a 57 kDa band corresponding to PDI was observed across the cell lines and tissues tested. Membrane enriched extracts (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane2), NIH/3T3 (Lane 3), A-375 (Lane 4), A-431 (Lane 5), HEK-293 (Lane 6), and tissue extracts of Mouse Liver (Lane 7), Rat Liver (lane 8) and Rat Testis (Lane 9) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nit... View More

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PDI Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A-431 cells were transfected with PDI siRNA and reduction of signal was observed in Western Blot using PDI Monoclonal Antibody (Product # PA5-77821). {KD}

产品信息

PA5-77821

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组织化学（多聚甲醛固定） (IHC (PFA))

1:1,000

免疫细胞化学 (ICC/IF)

1:100

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免疫沉淀 (IP)

Assay-Dependent

产品规格

种属反应

Bovine, Dog, Guinea pig, Hamster, Human, Mollusc, Mouse, Sheep, Pig, Rat, Xenopus

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Rat PDI synthetic peptide (409-509 aa C-terminal) conjugated to KLH

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography, Protein A

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.09% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2736334

产品详细信息

A 1:1,000 dilution of PA5-77821 was sufficient for detection of PDI in 20 µg of HeLa cell lysate by ECL immunoblot analysis.|Detects approximately 58kDa.

靶标信息

Protein disulfide isomerase (PDI) is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, the protein seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. It may therefore cause structural modifications of exofacial proteins. Inside the cell, PDI seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, it functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity). PDI may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Identification of signalling downstream of the transcription factor forkhead box protein M1 that protects against endoplasmic reticulum stress in a diabetic foot ulcer model.

Diabetic medicine : a journal of the British Diabetic Association

Peng W,Zhu T,Xiang G,Ding T,Zhao J,Xiong D,Zhong Y,Zhang Y

PA5-77821 was used in Immunocytochemistry to reveal that FOXM1 suppressed ER stress, cell apoptosis and promoted angiogenesis in HG-induced HUVECs via mediating GAS5/TAF15/SDF4 axis, providing a novel therapeutic molecule mechanism for DFU.

Thu Jun 01 00:00:00 UTC 2023

生物信息学

蛋白别名: CaO19.12595; cellular thyroid hormone binding protein; Cellular thyroid hormone-binding protein; collagen prolyl 4-hydroxylase beta; Endoplasmic reticulum resident protein 59; Eps1p; ER protein 59; glutathione-insulin transhydrogenase; p55; p58; pancreas-specific protein disulfide isomerase; PDI; PDI (E.C.5.3.4.1); PDI1P; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide; prolyl 4-hydroxylase beta polypeptide; Prolyl 4-hydroxylase subunit beta; prolyl 4-hydroxylase, beta polypeptide; prolyl 4-hydroxylase, beta subunit; protein disulfide isomerase; Protein disulfide isomerase (Prolyl 4-hydroxylase, beta polypeptide); protein disulfide isomerase A2; protein disulfide isomerase family A, member 1; protein disulfide isomerase family A, member 2; protein disulfide isomerase-associated 1; protein disulfide isomerase/oxidoreductase; Protein disulfide-isomerase; protocollagen hydroxylase; testicular secretory protein Li 32; thyroid hormone-binding protein p55

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基因别名: CLCRP1; DSI; ERBA2L; ERp59; GIT; I79_002399; P4HB; P4Hbeta; p58; PDI; PDIA1; pdia2; pdip; PDIR; PHDB; PO4DB; PO4HB; PROHB; Thbp; XELAEV_18043753mg; XELAEV_18045582mg; XPDIp