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PDK1 Antibody (MA5-32702) in ICC/IF
Immunofluorescence analysis of PDK1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PDK1 Recombinant Rabbit Monoclonal Antibody (Product # PA5-32702) at 1:100 in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjug... View More
产品信息
MA5-32702
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Not Applicable
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JA67-30
抗原
Synthetic peptide within Human PDK1 aa 16-65
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2809979
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
PDK1 (3-phosphoinositide dependent kinase-1) is a serine/threonine protein kinase localized to the plasma membrane. It plays a vital role in insulin-induced glycogen synthesis, PKC activation, and activation of the AGC subfamily of protein kinases. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial; isoenzyme 1; kinase isoenzyme 1; lipoamide; mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1; PDH kinase 1; PDK p48; pyruvate dehydrogenase; pyruvate dehydrogenase kinase 1; Pyruvate dehydrogenase kinase isoform 1; pyruvate dehydrogenase kinase, isoenzyme 1; pyruvate dehydrogenase kinase, isozyme 1
基因别名: B830012B01; D530020C15Rik; PDHK1; PDK1
UniProt ID: (Human) Q15118, (Mouse) Q8BFP9
Entrez Gene ID: (Human) 5163, (Rat) 116551, (Mouse) 228026
protein kinase activity
pyruvate dehydrogenase (acetyl-transferring) kinase activity
protein binding
ATP binding
protein serine/threonine kinase activity
protein complex binding
protein homodimerization activity
protein heterodimerization activity
nucleotide binding
kinase activity
transferase activity
non-receptor serine/threonine protein kinase
glucose metabolic process
protein phosphorylation
cell proliferation
intrinsic apoptotic signaling pathway in response to oxidative stress
regulation of acetyl-CoA biosynthetic process from pyruvate
regulation of glucose metabolic process
hypoxia-inducible factor-1alpha signaling pathway
mitophagy in response to mitochondrial depolarization
cell surface receptor signaling pathway
peptidyl-serine phosphorylation
carbohydrate metabolic process
phosphorylation
intracellular signal transduction
