Invitrogen

PDXDC1 Polyclonal Antibody

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抗体检测数据 (4)

PDXDC1 Antibody in Immunocytochemistry (ICC/IF)

PDXDC1 Antibody in Immunocytochemistry (ICC/IF)

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PDXDC1 Antibody (PA5-101798) in ICC/IF

Immunocytochemistry analysis of PDXDC1 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with PDXDC1 polyclonal antibody (Product # PA5-101798) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

PDXDC1 Antibody in Immunocytochemistry (ICC/IF)

PDXDC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PDXDC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PDXDC1 Antibody in Western Blot (WB)

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PDXDC1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of PDXDC1 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with PDXDC1 polyclonal antibody (Product # PA5-101798) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

PDXDC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PDXDC1 in mouse testis tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with PDXDC1 polyclonal antibody (Product # PA5-101798) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

PDXDC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PDXDC1 in rat testis tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with PDXDC1 polyclonal antibody (Product # PA5-101798) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

PDXDC1 Antibody in Western Blot (WB) — Western blot analysis of PDXDC1 in mouse brain (Lane 1: treated with blocking peptide). Samples were incubated with PDXDC1 polyclonal antibody (Product # PA5-101798).

产品信息

PA5-101798

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human PDXDC1(Accession Q6P996), corresponding to amino acid residues I676-I726.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2851230

产品详细信息

Antibody detects endogenous levels of total PDXDC1.

靶标信息

PDXDC1 (Pyridoxal Dependent Decarboxylase Domain Containing 1) is a Protein Coding gene. Diseases associated with PDXDC1 include Autosomal Recessive Nonsyndromic Deafness 32 and Fetal Akinesia Deformation Sequence 1. Gene Ontology (GO) annotations related to this gene include pyridoxal phosphate binding and carboxy-lyase activity. An important paralog of this gene is HDC. [GeneCards]

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: pyridoxal-dependent decarboxylase domain containing 1; Pyridoxal-dependent decarboxylase domain-containing protein 1

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基因别名: 2210010A19Rik; AA415817; KIAA0251; Kiaa0251-hp; LP8165; PDXDC1; RGD1562597

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UniProt ID: (Human) Q8TBS5, (Mouse) Q99K01

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Entrez Gene ID: (Human) 23042, (Mouse) 94184, (Rat) 304721

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carboxy-lyase activity pyridoxal phosphate binding cadherin binding involved in cell-cell adhesion catalytic activity phosphoenolpyruvate carboxykinase activity gamma-glutamyl carboxylase activity 3-octaprenyl-4-hydroxybenzoate carboxy-lyase activity lyase activity 2-hydroxy-3-carboxy-6-oxo-7-methylocta-2,4-dienoate decarboxylase activity bis(4-chlorophenyl)acetate decarboxylase activity 3,5-dibromo-4-hydroxybenzoate decarboxylase activity 2-hydroxyisobutyrate decarboxylase activity 2-hydroxy-2-methyl-1,3-dicarbonate decarboxylase activity 2-hydroxyisophthalate decarboxylase activity dimethylmalonate decarboxylase activity 2,4,4-trimethyl-3-oxopentanoate decarboxylase activity 4,4-dimethyl-3-oxopentanoate decarboxylase activity 2,3,6-trihydroxyisonicotinate decarboxylase activity phenanthrene-4,5-dicarboxylate decarboxylase activity pyrrole-2-carboxylate decarboxylase activity terephthalate decarboxylase activity malonate semialdehyde decarboxylase activity 5-amino-4-imidazole carboxylate lyase activity 2-keto-4-methylthiobutyrate aminotransferase activity 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase activity

carboxylic acid metabolic process cell-cell adhesion biological_process