Invitrogen

PDXK Polyclonal Antibody

PDXK Antibody in Immunocytochemistry (ICC/IF)

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PDXK Antibody (PA5-56393) in ICC/IF

Immunofluorecent analysis of PDXK in human cell line U-2 OS using PDXK Polyclonal Antibody (Product # PA5-56393). Staining shows localization to nucleoplasm.

PDXK Antibody in Immunohistochemistry (IHC)

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PDXK Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of PDXK demonstrates antibody specificity. Immunohistochemical analysis of PDXK using anti-PDXK Polyclonal Antibody (Product # PA5-56393), shows significant staining of PDXK in cerebral cortex and shows minimal or weak staining in skeletal muscle tissues. The relative expression levels of PDXK within each tissue is shown using RNA-Seq. {RE}

产品信息

PA5-56393

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

免疫组化（石蜡） (IHC (P))

1:1,000-1:2,500

免疫细胞化学 (ICC/IF)

0.25-2 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human PDXK. Recombinant protein control fragment (Product #RP-95173).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.3 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2645357

产品详细信息

Immunogen sequence: LLPVYKEKVV PLADIITPNQ FEAELLSGRK IHSQEEALRV MDMLHSMGPD TVVITSSDLP SPQGSNYLIV LGSQ

Highest antigen sequence identity to the following orthologs: Mouse - 88%, Rat - 86%.

靶标信息

Pyridoxal kinase (PDXK) converts vitamin B6 to pyridoxal-5-phosphate (PLP), an essential cofactor in the intermediate metabolism of amino acids and neurotransmitters. The PDXK gene encodes a 312-amino acid polypeptide, and expression of the cDNA reveals pyridoxal kinase activity. Northern blot analysis revealed that a major 1.5-kb PDXK transcript is expressed in all tissues tested. The expression of PDXK shows circadian oscillations. The expression of Pdxk in mouse liver and brain is regulated by the 3 PAR bZIP transcription factors, Dbp, Hlf, and Tef, which also show circadian oscillations in expression. Mice devoid of all 3 transcription factors show decreased levels of brain PLP, serotonin, and dopamine, and are highly susceptible to frequently lethal generalized spontaneous and audiogenic epilepsies.

仅用于科研。不用于诊断过程。未经明确授权不得转售。