Invitrogen

PDZD8 Polyclonal Antibody

图: 1 / 7

PDZD8 Antibody (PA5-46771) in WB

Western blot analysis of human fetal lung cell lysate using an anti-PDZD8 polyclonal antibody (Product # PA5-46771).

PDZD8 Antibody in Surface plasmon resonance (SPR)

Product Image Gallery

PDZD8 Antibody — Validation of MERLIN. (A, B, C, D) PDZD8 modulates ER-mitochondria distance.(A, B, C) Representative Western blot of the PDZD8 levels when transiently transfected and (B) upon silencing with siRNA_PDZD8 in HCT116 cells, whose quantification is shown in (C) (n = 3). (D) BRET signal in cells co-expressing Rluc-L1-B33C and Scal-L1-mVenus biosensor combination, in the presence of overexpressed PDZD8, the synthetic tether mTagBFP2 and PDZD8 knockdown in HCT116 cells. (**P < 0.025, ***P = 0.001). t test, data are expressed as mean ± SD. (E, F) The BRET signal of MERLIN is increased in apoptotic cells. (E) Confocal images of Cos1 cells transfected with sCal-L1-mVen (green) and RLuc-L1-B33C (magenta) under healthy condition and upon apoptosis induction with 1 µM STS at different times. Scale bar 10 µM. (F) Scheme and graph showing the change of the BRET signal in apoptotic cells over time for the 12-nm linker MERLIN. Black lines represent four individual measurements and the grey line the control measurement without induction of apoptosis. Apoptosis was induced at time point 0 h by addition of 1 µM STS. (N = 4). (G) MERLIN detects a NAC-induced decrease in MERCs (**P < 0.025) t test, data are expressed as mean ± SD. {KD}

产品信息

PA5-46771

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

查看2篇已发表文章 2篇已发表文章

Surface plasmon resonance (SPR)

Assay-dependent

产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide directed towards the C-terminal of human PDZD8 (aa 935-984).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS with 2% sucrose

内含物

0.09% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2606549

产品详细信息

Peptide sequence: TRHIINTSSR LLNLRQVSKT RLSEPGTDLV EPSPKHTPNT SDNEGSDTEV

靶标信息

PDZD8 plays a role in the regulation of cell morphology and cytoskeletal organization. It may inhibit herpes simplex virus 1 infection at an early stage and may promote retroviral infection.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

circ_0020123 promotes cell proliferation and migration in lung adenocarcinoma via PDZD8.

Open medicine (Warsaw, Poland)

Wei W,Wang C,Wang L,Zhang J

Published figure using PDZD8 polyclonal antibody (Product # PA5-46771) in Western Blot

Thu Apr 14 00:00:00 UTC 2022

MERLIN: a novel BRET-based proximity biosensor for studying mitochondria-ER contact sites.

Life science alliance

Hertlein V,Flores-Romero H,Das KK,Fischer S,Heunemann M,Calleja-Felipe M,Knafo S,Hipp K,Harter K,Fitzgerald JC,García-Sáez AJ

Published figure using PDZD8 polyclonal antibody (Product # PA5-46771) in Western Blot

Wed Jan 01 00:00:00 UTC 2020

生物信息学

蛋白别名: bA129M16.2; FLJ34427; PDZ domain-containing protein 8; Sarcoma antigen NY-SAR-84/NY-SAR-104

查看更多收起

基因别名: PDZD8; PDZK8

查看更多收起

UniProt ID: (Human) Q8NEN9

查看更多收起

Entrez Gene ID: (Human) 118987

查看更多收起

功能

metal ion binding scaffold/adaptor protein

参与通路

cytoskeleton organization viral process regulation of cell morphogenesis intracellular signal transduction

PDZD8 Polyclonal Antibody

PDZD8 Antibody (PA5-46771) in WB

产品信息

PA5-46771

免疫印迹 (WB)

Surface plasmon resonance (SPR)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!