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PDZK1 Antibody (PA5-102727) in ICC/IF
Immunocytochemistry analysis of PDZK1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with PDZK1 polyclonal antibody (Product # PA5-102727) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-102727
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PDZK1(Accession Q5T2W1), corresponding to amino acid residues P482-M519.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2852118
Product Specific Information
Antibody detects endogenous levels of total PDZK1.
Target Information
PDZK1 is a scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. It may be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, it may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. It may also play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. PDZK1 functions to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism and may be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-terminal linking and modulating protein; C-terminal-linking and modulating protein; CFTR-associated protein of 70 kDa; Dietary Pi-regulated RNA-1; Diphor-1; Na(+)/H(+) exchange regulatory cofactor NHE-RF3; Na(+)/H(+) exchanger regulatory factor 3; Na/Pi cotransporter C-terminal-associated protein 1; NaPi-C; NaPi-Cap1; NHERF-3; PDZ domain-containing protein 1; PDZ-containing kidney protein 1; PDZK 1; PDZK-1; Sodium-hydrogen exchanger regulatory factor 3
Gene Aliases: 1700023D20Rik; 2610507N21Rik; 4921513F16Rik; AI267131; AI314638; AL022680; CAP70; CLAMP; D3Ertd537e; Diphor1; mPDZK1; NHERF-3; NHERF3; PDZD1; PDZK1
UniProt ID: (Human) Q5T2W1, (Mouse) Q9JIL4, (Rat) Q9JJ40
Entrez Gene ID: (Human) 5174, (Mouse) 59020, (Rat) 65144
transport
cell proliferation
carnitine transport
drug transport
positive regulation of ion transmembrane transport
regulation of anion transport
positive regulation of protein targeting to membrane
establishment of protein localization to plasma membrane
cholesterol transport
intracellular transport
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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