Invitrogen

PER1 Polyclonal Antibody

PER1 Antibody in Immunocytochemistry (ICC/IF)

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PER1 Antibody (PA1-524) in ICC/IF

Immunofluorescent analysis of PER1 (green) showing staining in the cytoplasm and nucleus of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a PER1 polyclonal antibody (Product # PA1-524) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyL... View More

PER1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PER1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

PER1 Antibody — Fig. 4 Knockdown of PER1 in the dorsal hippocampus impairs long-term memory. Per1 mRNA expression is upregulated 60 m in the young hippocampus after a OLM ( t (8) = 4.49, ** p < 0.01; n = 5, 5; all male) and b context fear conditioning ( t (8) = 3.01, *p < 0.05, n = 5, 5; all male). c Top, schematic of ChIP primer sites along the Per1 promoter. Bottom, HDAC3 ChIP results. HDAC3 occupancy was reduced along the Per1 promoter in the young hippocampus after OLM (Training effect only, F (1,21) = 8.51, ** p < 0.01, n = 4, 5; all male). d Hippocampal PER1 protein expression was significantly reduced by Per1 siRNA infusion ( t (20) = 2.72, * p = 0.01, n = 11,11; all male). e Per1 knockdown impaired OLM (Two-way ANOVA: siRNA x Session ( F (1,19) = 29.22, p < 0.001), Sidak's post hoc tests, *** p < 0.001, n = 11, 10; all male). f Per1 siRNA did not affect total exploration time at test ( t (19) = 0.27, p = 0.79). Data are presented as mean +- SEM {KD}

产品信息

PA1-524

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:1,000

查看11篇已发表文章 11篇已发表文章

免疫组化 (IHC)

查看8篇已发表文章 8篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:500

免疫组化（冰冻） (IHC (F))

1:50-1:500

免疫细胞化学 (ICC/IF)

1:10-1:200

查看3篇已发表文章 3篇已发表文章

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

其他PubMed (MISC)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Fruit fly, Hamster, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic Peptide: P(39) S L A D D T D A N S N G(51) C

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2268030

产品详细信息

PA1-524 detects PER1 from human and mouse tissues as well as recombinant mouse PER1.

PA1-524 has been sucessfully used in Western blot, immunofluoresence and immunohistochemistry procedures. By Western blot, this antibody detects an ~136 kDa protein representing overexpressed recombinant mouse PER1 protein. Immunofluorescence staining of PER1 in mouse testes with PA1-524 results in cytoplasmic staining.

PA1-524 immunizing peptide corresponds to amino acid residues 39-51 from mouse PER1. PA1-524 immunizing peptide (Cat. # PEP-076) is available for use in neutralization and control experiments.

靶标信息

Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein-mediated, autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-ARNT3 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK has been shown to transactivate the mammalian homolog of Drosophila per. PER, in concert with the product of the mammalian timeless gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Inhibition of casein kinase 1δ/εimproves cognitive-affective behavior and reduces amyloid load in the APP-PS1 mouse model of Alzheimer's disease.

Scientific reports

Sundaram S,Nagaraj S,Mahoney H,Portugues A,Li W,Millsaps K,Faulkner J,Yunus A,Burns C,Bloom C,Said M,Pinto L,Azam S,Flores M,Henriksen A,Gamsby J,Gulick D

PA1-524 was used in Immunohistochemistry-immunofluorescence to examine how inhibition of CK1ε/δ with PF-670462 impacts regional Aβ and circadian gene expression.

Tue Sep 24 00:00:00 UTC 2019

Inhibition of casein kinase 1δ/εimproves cognitive-affective behavior and reduces amyloid load in the APP-PS1 mouse model of Alzheimer's disease.

Scientific reports

Sundaram S,Nagaraj S,Mahoney H,Portugues A,Li W,Millsaps K,Faulkner J,Yunus A,Burns C,Bloom C,Said M,Pinto L,Azam S,Flores M,Henriksen A,Gamsby J,Gulick D

PA1-524 was used in Immunohistochemistry-immunofluorescence to examine how inhibition of CK1ε/δ with PF-670462 impacts regional Aβ and circadian gene expression.

Tue Sep 24 00:00:00 UTC 2019

Epigenetic regulation of the circadian gene Per1 contributes to age-related changes in hippocampal memory.

Nature communications

Kwapis JL,Alaghband Y,Kramár EA,López AJ,Vogel Ciernia A,White AO,Shu G,Rhee D,Michael CM,Montellier E,Liu Y,Magnan CN,Chen S,Sassone-Corsi P,Baldi P,Matheos DP,Wood MA

PA1-524 was used in Western Blotting to investigate whether age-related disruption of the circadian clock causes impaired hippocampal memory.

Mon Aug 20 00:00:00 UTC 2018

FAD Regulates CRYPTOCHROME Protein Stability and Circadian Clock in Mice.

Cell reports

Hirano A,Braas D,Fu YH,Ptáček LJ

PA1524 was used in western blot to investigate the role of flavin adenine dinucleotide as a mediator of the clock and metabolism

Tue Apr 11 00:00:00 UTC 2017

A Cryptochrome 2 mutation yields advanced sleep phase in humans.

eLife

Hirano A,Shi G,Jones CR,Lipzen A,Pennacchio LA,Xu Y,Hallows WC,McMahon T,Yamazaki M,Ptáček LJ,Fu YH

PA1524 was used in western blot to show that stability of cryptochrome 2 has an important role in regulating human sleep wake behavior

Tue Aug 16 00:00:00 UTC 2016

The Circadian Clock Gene Period1 Connects the Molecular Clock to Neural Activity in the Suprachiasmatic Nucleus.

ASN neuro

Kudo T,Block GD,Colwell CS

PA1-524 was used in western blot to investigate how Period1 regulates the electrical activity in the mouse suprachiasmatic nucleus

Fri Aug 12 00:00:00 UTC 2016

Specificity in circadian clock feedback from targeted reconstitution of the NuRD corepressor.

Molecular cell

Kim JY,Kwak PB,Weitz CJ

PA1-524 was used in western blot to investigate how different NuRD subunits regulate the PER complex.

Thu Dec 18 00:00:00 UTC 2014

Modulation of mammalian circadian rhythms by tumor necrosis factor-α.

Chronobiology international

Paladino N,Mul Fedele ML,Duhart JM,Marpegan L,Golombek DA

PA1-524 was used in immunohistochemistry to study the mechanism by which TNF-alpha modulates the mammalian circadian rhythm

Sun Jun 01 00:00:00 UTC 2014

A role for the circadian clock protein Per1 in the regulation of the NaCl co-transporter (NCC) and the with-no-lysine kinase (WNK) cascade in mouse distal convoluted tubule cells.

The Journal of biological chemistry

Richards J,Ko B,All S,Cheng KY,Hoover RS,Gumz ML

PA1-524 was used in immunoprecipitation and western blot to study the transcriptional regulation NaCl co-transporter, WNK1 and WNK4 expression in the murine distal convoluted tubule by the Per1 clock protein

Fri Apr 25 00:00:00 UTC 2014

Opposing actions of Per1 and Cry2 in the regulation of Per1 target gene expression in the liver and kidney.

American journal of physiology. Regulatory, integrative and comparative physiology

Richards J,All S,Skopis G,Cheng KY,Compton B,Srialluri N,Stow L,Jeffers LA,Gumz ML

PA1-524 was used in immunoprecipitation and western blot to study the expression of Per1 target genes in liver and kidney and the opposing effects of Per1 and Cry2

Tue Oct 01 00:00:00 UTC 2013

Human epidermal stem cell function is regulated by circadian oscillations.

Cell stem cell

Janich P,Toufighi K,Solanas G,Luis NM,Minkwitz S,Serrano L,Lehner B,Benitah SA

PA1-524 was used in western blot to study the role of circadian rhythms in modulating the function of human skin stem cells

Thu Dec 05 00:00:00 UTC 2013

Role of Per1 and the mineralocorticoid receptor in the coordinate regulation of αENaC in renal cortical collecting duct cells.

Frontiers in physiology

Richards J,Jeffers LA,All SC,Cheng KY,Gumz ML

PA1-524 was used in ChIP assay and western blot to study the mechanisms underlying the regulation of the alpha-ENaC aldosterone response by Per1 and the mineralocorticoid receptor

Tue Sep 24 00:00:00 UTC 2013

Occurrence of epilepsy at different zeitgeber times alters sleep homeostasis differently in rats.

Sleep

Yi PL,Chen YJ,Lin CT,Chang FC

PA1-524 was used in Immunohistochemistry to suggest that the occurrence of epilepsy at different zeitgeber times alters sleep processes differently.

Sat Dec 01 00:00:00 UTC 2012

cGMP-phosphodiesterase inhibition enhances photic responses and synchronization of the biological circadian clock in rodents.

PloS one

Plano SA,Agostino PV,de la Iglesia HO,Golombek DA

PA1-524 was used in immunohistochemistry to study the role of the cGMP pathway in the response of the rodent circadian clock to light

Wed Sep 19 00:00:00 UTC 2012

Circadian entrainment to light-dark cycles involves extracellular nitric oxide communication within the suprachiasmatic nuclei.

The European journal of neuroscience

Plano SA,Golombek DA,Chiesa JJ

PA1-524 was used in immunohistochemistry to investigate the mechanism of circadian entrainment to light-dark cycles

Mon Mar 01 00:00:00 UTC 2010

Regulation of αENaC expression by the circadian clock protein Period 1 in mpkCCD(c14) cells.

Biochimica et biophysica acta

Gumz ML,Cheng KY,Lynch IJ,Stow LR,Greenlee MM,Cain BD,Wingo CS

PA1-524 was used in western blot to study the role of the circadian clock protein Period1 in regulating ENaC.

Wed Sep 01 00:00:00 UTC 2010

Regulation of αENaC expression by the circadian clock protein Period 1 in mpkCCD(c14) cells.

Biochimica et biophysica acta

Gumz ML,Cheng KY,Lynch IJ,Stow LR,Greenlee MM,Cain BD,Wingo CS

PA1-524 was used in western blot to study the role of the circadian clock protein Period1 in regulating ENaC.

Wed Sep 01 00:00:00 UTC 2010

Loss of melatonin signalling and its impact on circadian rhythms in mouse organs regulating blood glucose.

European journal of pharmacology

Mühlbauer E,Gross E,Labucay K,Wolgast S,Peschke E

PA1-524 was used in western blot to investigate the importance of melatonin in circadian rhythms in mouse pancreas and liver

Sun Mar 15 00:00:00 UTC 2009

Up-regulation of Per1 expression by estradiol and progesterone in the rat uterus.

The Journal of endocrinology

He PJ,Hirata M,Yamauchi N,Hattori MA

PA1-524 was used in immunocytochemistry to study the effect of estradiol and progesterone on Per1 expression in the rat uterus

Sat Sep 01 00:00:00 UTC 2007

Constitutive expression of the Period1 gene impairs behavioral and molecular circadian rhythms.

Proceedings of the National Academy of Sciences of the United States of America

Numano R,Yamazaki S,Umeda N,Samura T,Sujino M,Takahashi R,Ueda M,Mori A,Yamada K,Sakaki Y,Inouye ST,Menaker M,Tei H

PA1-524 was used in immunohistochemistry to investigate the impact of period1 gene on behavioral and molecular circadian rhythms.

Tue Mar 07 00:00:00 UTC 2006

Indication of circadian oscillations in the rat pancreas.

FEBS letters

Mühlbauer E,Wolgast S,Finckh U,Peschke D,Peschke E

PA1-524 was used in western blot to investigate peripheral circadian oscillation in rat pancreas

Fri Apr 23 00:00:00 UTC 2004

Expression of circadian rhythm genes in gonadotropin-releasing hormone-secreting GT1-7 neurons.

Endocrinology

Gillespie JM,Chan BP,Roy D,Cai F,Belsham DD

PA1-524 was used in western blot to investigate the role of the clock machinery in GnRH neuronal function.

Mon Dec 01 00:00:00 UTC 2003

Non-cyclic and developmental stage-specific expression of circadian clock proteins during murine spermatogenesis.

Biology of reproduction

Alvarez JD,Chen D,Storer E,Sehgal A

PA1-524 was used in immunohistochemistry to investigate the expression of circadian clock proteins during murine spermatogenesis

Tue Jul 01 00:00:00 UTC 2003

Period gene expression in mouse endocrine tissues.

American journal of physiology. Regulatory, integrative and comparative physiology

Bittman EL,Doherty L,Huang L,Paroskie A

PA1-524 was used in immunocytochemistry to study the expression of PER1 in mouse endocrine tissues

Mon Sep 01 00:00:00 UTC 2003

Restoration of circadian behavioural rhythms in a period null Drosophila mutant (per01) by mammalian period homologues mPer1 and mPer2.

Genes to cells : devoted to molecular & cellular mechanisms

Shigeyoshi Y,Meyer-Bernstein E,Yagita K,Fu W,Chen Y,Takumi T,Schotland P,Sehgal A,Okamura H

PA1-524 was used in immunohistochemistry to investigate the role of period gene in the generation of circadian rhythm

Fri Feb 01 00:00:00 UTC 2002

生物信息学

蛋白别名: Circadian clock protein PERIOD 1; Circadian pacemaker protein Rigui; hPER1; mPER1; Period circadian protein homolog 1; period homolog 1; Period, drosophila, homolog of

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基因别名: hPER; KIAA0482; m-rigui; mPer1; PER; PER1; RIGUI

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UniProt ID: (Human) O15534, (Mouse) O35973

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Entrez Gene ID: (Human) 5187, (Mouse) 18626

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功能