Invitrogen

PER1 Polyclonal Antibody

PER1 Antibody in Immunocytochemistry (ICC/IF)

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PER1 Antibody (PA5-119798) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of of PER1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with PER1 Polyclonal Antibody (Product # PA5-119798) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).

PER1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-119798

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:100-1:500

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein within human PER1 aa 320-519

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

TBS, pH 7.4, with 50% glycerol, 0.2% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2913370

产品详细信息

Positive Control: 293T cell lysate, Hela cell lysate, Daudi cell lysate, mouse pancreas tissue lysate, rat brain tissue lysate, Hela, rat kidney tissue.

Subcellular Location: Nucleus, Cytoplasm.

靶标信息

Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein-mediated, autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-ARNT3 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK has been shown to transactivate the mammalian homolog of Drosophila per. PER, in concert with the product of the mammalian timeless gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Circadian clock protein PERIOD 1; Circadian pacemaker protein Rigui; hPER1; mPER1; per1 {ECO:0000312|RGD:727863}; period 1; Period circadian protein homolog 1; period homolog 1; Period, drosophila, homolog of; rPER1

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基因别名: hPER; KIAA0482; m-rigui; mPer1; PER; PER1; RIGUI; rper1

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UniProt ID: (Human) O15534, (Mouse) O35973, (Rat) Q8CHI5

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Entrez Gene ID: (Human) 5187, (Mouse) 18626, (Rat) 287422

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功能