Invitrogen

PF4 Polyclonal Antibody, Biotin, PeproTech®

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PF4 Antibody (500-P05BT-25UG) in WB

Western Blot: To detect hPF-4 by Western Blot analysis PF4 Polyclonal Antibody, Biotin (Product # 500-P05BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hPF-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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产品信息

500-P05BT-25UG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

酶联免疫吸附实验 (ELISA)

0.25-1.0 µg/mL

免疫染色 (IS)

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产品规格

种属反应

Human

已发表种属

Mouse

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived, 7.8 kDa Recombinant Human PF-4 (CXCL4)

查看免疫原

偶联物

Biotin Biotin Biotin

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

AB_2929163

产品详细信息

AA Sequence of recombinant protein: EAEEDGDLQC LCVKTTSQVR PRHITSLEVI KAGPHCPTAQ LIATLKNGRK ICLDLQAPLY KKIIKKLLES.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human PF-4 (CXCL4). Anti-Human PF-4 (CXCL4)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Human PF-4 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human PF-4 (500-P05) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human PF-4.

Western Blot: To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hPF-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P05BT-1MG will be provided as 2 x 500 µg

靶标信息

Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair (Eisman et al., 1990).

仅用于科研。不用于诊断过程。未经明确授权不得转售。