Invitrogen

PF4 Monoclonal Antibody (140910)

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PF4 Antibody (MA5-23868) in WB

Western blot analysis of PF4 in mouse platelets. Samples were incubated in PF4 monoclonal antibody (Product # MA5-23868) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Rat IgG secondary antibody. A specific band was detected for CXCL4/PF4 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions.

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产品信息

MA5-23868

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

酶联免疫吸附实验 (ELISA)

0.1-0.4 µg/mL (Detection), 2-8 µg/mL (Capture)

产品规格

种属反应

Mouse

宿主/亚型

Rat / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

140910

抗原

E. coli-derived recombinant mouse CXCL4/PF4 Val30-Ser105

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.5 mg/mL

纯化类型

Protein A/G

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2576249

产品详细信息

In ELISAs, this antibody does not cross-react or interfere recombinant human (rh) CXCL/PF4, recombinant mouse (rm) CXCL9/MIG, rmGM-CSF, rmCXCL11/I-TAC, rhCXCL8/IL-8, rmIL-5, or rhCXCL10/IP-10. In Western blots, no cross-reactivity was observed with rhGCP-2, rmGCP-2, rhGRO alpha, rhGRO beta, rhGRO gamma, rhMIP-1 alpha, rmMIP-1 alpha, rmMIP-1 beta, rhMIP-1 gamma, rmMIP-1 gamma, rmMIP-2, rhMIP-3 alpha, rmMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, or rmMIP-3 beta,

Reconstitute at 0.5 mg/mL in sterile PBS.

靶标信息

Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair (Eisman et al., 1990).

仅用于科研。不用于诊断过程。未经明确授权不得转售。