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产品信息
PA5-21685
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 428 and 674 of PFKL (Uniprot ID#P17858)
偶联物
形式
Liquid
浓度
1.23 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_11155219
产品详细信息
Recommended positive controls: A431, H1299, HeLa, HepG2.
Predicted reactivity: Mouse (95%), Rat (95%), Chicken (88%), Bovine (94%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Phosphofructokinase (PFK), a major regulatory enzyme in all cells of the body, catalyzes the metabolism of sugar, and thereby is pivotal in the production of energy to maintain normal cell function. In human there are three structural loci controlling PFK: M (muscle), L (liver), and P (platelet) type subunits, which are variably expressed in different tissues; human diploid fibroblasts and leukocytes express all three genes. PFK, a tetramer formed by the random association of the products of two separate gene loci to form the five possible tetramers. PFKs of muscle and liver are homotetramers of the M and L subunits, respectively. Red cells have all five isozymes: M4, M3L, M2L2, ML3, and L4. PFK is an allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. PFK catalyzes the key controlling step of glycolytic pathway. PFK deficiency can present as mild to life-threatening episodic illness. A hallmark sign of this disease is intermittent dark urine, with the color of the urine ranging from orange to dark coffee-brown, which commonly develops following strenuous exercise. The mean red cell PFK is elevated in persons with Down syndrome.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 6-phosphofructokinase type B; 6-phosphofructokinase, liver type; ATP-dependent 6-phosphofructokinase, liver type; ATP-PFK; EC 2.7.1.11; K6PL; liver-type 1-phosphofructokinase; PFK-B; Phosphofructo-1-kinase isozyme B; phosphofructokinase 1; Phosphohexokinase
基因别名: AA407869; ATP-PFK; PFK-B; PFK-L; Pfkb; PFKL
UniProt ID: (Human) P17858, (Mouse) P12382
Entrez Gene ID: (Human) 5211, (Mouse) 18641
6-phosphofructokinase activity
protein binding
ATP binding
kinase binding
identical protein binding
metal ion binding
fructose binding
fructose-6-phosphate binding
nucleotide binding
catalytic activity
kinase activity
transferase activity
monosaccharide binding
carbohydrate kinase
kinase
transferase
metabolite interconversion enzyme
fructose 6-phosphate metabolic process
glycolytic process
response to glucose
fructose 1,6-bisphosphate metabolic process
negative regulation of insulin secretion
protein oligomerization
protein homotetramerization
canonical glycolysis
carbohydrate metabolic process
metabolic process
phosphorylation
carbohydrate phosphorylation
