Invitrogen

PGC Polyclonal Antibody

PGC Antibody in Immunohistochemistry (IHC)

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PGC Antibody (PA5-56830) in IHC

Immunohistochemical staining of PGC in human cerebral cortex, colon, skeletal muscle and stomach using PGC Polyclonal Antibody (Product # PA5-56830) (A) shows similar protein distribution across tissues to an independent PGC Polyclonal Antibody (B).

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PGC Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of PGC demonstrates antibody specificity. Immunohistochemical analysis of PGC using anti-PGC Polyclonal Antibody (Product # PA5-56830), shows significant staining of PGC in human stomach and shows minimal or weak staining in human skeletal muscle tissues. The relative expression levels of PGC within each tissue is shown using RNA-Seq. {RE}

产品信息

PA5-56830

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

免疫组化（石蜡） (IHC (P))

1:50-1:200

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human PGC. Recombinant protein control fragment (Product #RP-94664).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2645441

产品详细信息

Immunogen sequence: VPLKKFKSIR ETMKEKGLLG EFLRTHKYDP AWKYRFGDLS VTYEPMAYMD AAYFGEISIG T

Highest antigen sequence identity to the following orthologs: Mouse - 73%, Rat - 75%.

靶标信息

Pepsin is one of the main proteolytic enzymes secreted by the gastric mucosa. Pepsin consists of a single polypeptide chain and arises from its precursor, pepsinogen, by removal of a 41 amino acid segment from the N-terminus. Pepsinogen is synthesized in the stomach lining, and hydrochloric acid, also produced by the gastric mucosa, is necessary to convert the inactive enzyme and to maintain the optimum acidity (pH 1-3) for pepsin function. Pepsin is particularly effective in cleaving peptide bonds involving aromatic amino acids. Pepsin shows extremely broad specificity, and although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. The amino acid composition of Pepsin C differs from those of pepsinogen and pepsin especially in the content of basic amino acids, glutamic acid, aspartic acid, leucine and isoleucine.

仅用于科研。不用于诊断过程。未经明确授权不得转售。