Invitrogen

PGP9.5 Polyclonal Antibody

PGP9.5 Antibody in Immunocytochemistry (ICC/IF)

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PGP9.5 Antibody (PA1-10011) in ICC/IF

Immunofluorescent analysis of PGP9.5 in cortical neuron-glial cell culture. The culture was prepared from an E20 rat and stained using a PGP9.5 polyclonal antibody (Product # PA1-10011) at a dilution of 1:500 as seen in red, and costained using a Vimentin monoclonal antibody at a dilution 1:2,000 as seen in green, and with DAPI staining the nuclear DNA in blue. The PGP9.5 antibody produces strong staining of the cell body and dendrites in neurons. The vimentin antibody stains intermediate filaments in fibroblastic and developing glial cell... View More

PGP9.5 Antibody in Immunohistochemistry (IHC)

PGP9.5 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

PGP9.5 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

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产品信息

PA1-10011

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000-1:5,000

免疫组化 (IHC)

查看7篇已发表文章 7篇已发表文章

免疫组化（石蜡） (IHC (P))

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免疫组织化学（多聚甲醛固定） (IHC (PFA))

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免疫组化（冰冻） (IHC (F))

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免疫细胞化学 (ICC/IF)

1:500-1:1,000

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产品规格

种属反应

Bovine, Horse, Human, Mouse, Pig, Rat

已发表种属

Cynomolgus monkey, Horse, Human, Mouse, Rat, Rhesus monkey

宿主/亚型

Chicken / IgY

分类

Polyclonal

类型

Antibody

抗原

Recombinant full length human UCHL1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

PBS

内含物

0.02% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_1088162

产品详细信息

PA1-10011 was made against full length recombinant human UCHL1 expressed in and purified from E. coli and can be used to identify neurons and their processes in culture or in sections. The antibody works cleanly on appropriate lysates of cell and tissues. |Stable at 4°C.

靶标信息

PGP9.5 (Protein gene product 9.5, UCH-L1, PARK5) is a neuron specific protein, structurally and immunologically distinct from neuron specific enolase. PGP9.5 has a molecular weight of 27 kDa and was first identified by high resolution two dimensional PAGE. PGP9.5 is a member of ubiquitin carboxyl-terminal hydrolase family 1 (peptidase family C12) with a ubiquitin carboxyl-terminal hydrolase domain. PGP9.5 is well known for having ubiquitin hydrolase and ligase activities that hydrolyzes small C-terminal adducts of ubiquitin to generate ubiquitin monomers. PGP9.5 is present in neurons and nerve fibers at all levels of the central and peripheral nervous system, in neuroendocrine cells, in segments of the renal tubules, in spermatogonia and Leydig cells of the testis, in ova and in some cells of both the pregnant and non-pregnant corpus luteum. Over expression of PGP9.5 leads to non-small cell lung cancer while decreased expression leads to Huntington disease and Alzheimer disease. Since PGP9.5 is present in cellular inclusions, it can be a useful as a neuronal marker and in the studies of neurodegenerative disorders such as with Parkinson disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Methods to Study the Myenteric Plexus of Rat Small Intestine.

Cellular and molecular neurobiology

Hecking I,Stegemann LN,Stahlke S,Theis V,Vorgerd M,Matschke V,Theiss C

PA1-10011 was used in Immunohistochemistry to present a new protocol for isolation and sample collection of rat myenteric plexus cells for in vivo and in vitro studies.

Sun Jan 01 00:00:00 UTC 2023

Progesterone: A Neuroprotective Steroid of the Intestine.

Cells

Stegemann LN,Neufeld PM,Hecking I,Vorgerd M,Matschke V,Stahlke S,Theiss C

PA1-10011 was used in Immunohistochemistry (Frozen) to investigate the potential neuroprotective effects of progesterone in the enteric nervous system (ENS), demonstrating its ability to reduce cell death in ENS neurons subjected to damage typical of Parkinson's disease.

Fri Apr 21 00:00:00 UTC 2023

The cannabinoid receptors system in horses: Tissue distribution and cellular identification in skin.

Journal of veterinary internal medicine

Kupczyk P,Rykala M,Serek P,Pawlak A,Slowikowski B,Holysz M,Chodaczek G,Madej JP,Ziolkowski P,Niedzwiedz A

PA1-10011 was used in Immunohistochemistry to represent a starting point for developing and translating new veterinary medicine-based pharmacotherapies using ECS as a possible target.

Fri Jul 01 00:00:00 UTC 2022

Oxidative DNA Damage and Cisplatin Neurotoxicity Is Exacerbated by Inhibition of OGG1 Glycosylase Activity and APE1 Endonuclease Activity in Sensory Neurons.

International journal of molecular sciences

Behrouzi A,Xia H,Thompson EL,Kelley MR,Fehrenbacher JC

PA1-10011 was used in Immunocytochemistry to investigate whether inhibiting OGG1 glycosylase activity with the small molecule inhibitor, TH5487, and/or APE1/Ref-1 endonuclease activity with APE Repair Inhibitor III would alter the neurotoxic effects of cisplatin in sensory neuronal cultures.

Tue Feb 08 00:00:00 UTC 2022

Oxidative DNA Damage and Cisplatin Neurotoxicity Is Exacerbated by Inhibition of OGG1 Glycosylase Activity and APE1 Endonuclease Activity in Sensory Neurons.

International journal of molecular sciences

Behrouzi A,Xia H,Thompson EL,Kelley MR,Fehrenbacher JC

Tue Feb 08 00:00:00 UTC 2022

Age-dependent increase of cytoskeletal components in sensory axons in human skin.

Frontiers in cell and developmental biology

Metzner K,Darawsha O,Wang M,Gaur N,Cheng Y,Rödiger A,Frahm C,Witte OW,Perocchi F,Axer H,Grosskreutz J,Brill MS

PA1-10011 was used in Immunohistochemistry to suggest gender-specific changes during aging in peripheral sensory axons, possibly influencing cytoskeletal functionality and axonal caliber.

Sat Nov 19 00:00:00 UTC 2022

Age-dependent increase of cytoskeletal components in sensory axons in human skin.

Frontiers in cell and developmental biology

Metzner K,Darawsha O,Wang M,Gaur N,Cheng Y,Rödiger A,Frahm C,Witte OW,Perocchi F,Axer H,Grosskreutz J,Brill MS

PA1-10011 was used in Immunohistochemistry to suggest gender-specific changes during aging in peripheral sensory axons, possibly influencing cytoskeletal functionality and axonal caliber.

Sat Nov 19 00:00:00 UTC 2022

Neuroprotective Effects of VEGF in the Enteric Nervous System.

International journal of molecular sciences

Hecking I,Stegemann LN,Theis V,Vorgerd M,Matschke V,Stahlke S,Theiss C

PA1-10011 was used in Immunohistochemistry (Frozen) to suggest a promising approach regarding neuroprotection, as the use of VEGF (may) prevent neuronal damage in the ENS.

Fri Jun 17 00:00:00 UTC 2022

Engineered AAVs for non-invasive gene delivery to rodent and non-human primate nervous systems.

Neuron

Chen X,Ravindra Kumar S,Adams CD,Yang D,Wang T,Wolfe DA,Arokiaraj CM,Ngo V,Campos LJ,Griffiths JA,Ichiki T,Mazmanian SK,Osborne PB,Keast JR,Miller CT,Fox AS,Chiu IM,Gradinaru V

PA1-10011 was used in Immunohistochemistry to study adeno-associated virus serotype 9 (AAV9) capsid in mice.

Wed Jul 20 00:00:00 UTC 2022

Engineered AAVs for non-invasive gene delivery to rodent and non-human primate nervous systems.

Neuron

Chen X,Ravindra Kumar S,Adams CD,Yang D,Wang T,Wolfe DA,Arokiaraj CM,Ngo V,Campos LJ,Griffiths JA,Ichiki T,Mazmanian SK,Osborne PB,Keast JR,Miller CT,Fox AS,Chiu IM,Gradinaru V

PA1-10011 was used in Immunohistochemistry to study adeno-associated virus serotype 9 (AAV9) capsid in mice.

Wed Jul 20 00:00:00 UTC 2022

Sex-specific differences in metabolic outcomes after sleeve gastrectomy and intermittent fasting in obese middle-aged mice.

American journal of physiology. Endocrinology and metabolism

Emiliano AB,Lopatinsky NR,Kraljević M,Higuchi S,He Y,Haeusler RA,Schwartz GJ

PA1-10011 was used in Immunohistochemistry to demonstrate that in middle-aged obese mice, female sex is associated with better metabolic outcomes after SG or IF with calorie restriction.

Fri Jul 01 00:00:00 UTC 2022

Alpha-synuclein and tau are abundantly expressed in the ENS of the human appendix and monkey cecum.

PloS one

Zinnen AD,Vichich J,Metzger JM,Gambardella JC,Bondarenko V,Simmons HA,Emborg ME

PA1-10011 was used in Immunocytochemistry, Western Blot, Immunohistochemistry to provide critical translational evidence that the common marmoset and rhesus macaque ceca are remarkably similar to the human appendix and, thus, that these NHP species are suitable for studying the development of PD linked to a-syn and tau pathological changes in the ENS.

Tue Jun 14 00:00:00 UTC 2022

Activation of GPR18 by Resolvin D2 Relieves Pain and Improves Bladder Function in Cyclophosphamide-Induced Cystitis Through Inhibiting TRPV1.

Drug design, development and therapy

Lu Q,Yang Y,Zhang H,Chen C,Zhao J,Yang Z,Fan Y,Li L,Feng H,Zhu J,Yi S

Published figure using PGP9.5 polyclonal antibody (Product # PA1-10011) in Immunocytochemistry

Wed Mar 02 00:00:00 UTC 2022

Activation of GPR18 by Resolvin D2 Relieves Pain and Improves Bladder Function in Cyclophosphamide-Induced Cystitis Through Inhibiting TRPV1.

Drug design, development and therapy

Lu Q,Yang Y,Zhang H,Chen C,Zhao J,Yang Z,Fan Y,Li L,Feng H,Zhu J,Yi S

Published figure using PGP9.5 polyclonal antibody (Product # PA1-10011) in Immunocytochemistry

Wed Mar 02 00:00:00 UTC 2022

Activation of GPR18 by Resolvin D2 Relieves Pain and Improves Bladder Function in Cyclophosphamide-Induced Cystitis Through Inhibiting TRPV1.

Drug design, development and therapy

Lu Q,Yang Y,Zhang H,Chen C,Zhao J,Yang Z,Fan Y,Li L,Feng H,Zhu J,Yi S

Published figure using PGP9.5 polyclonal antibody (Product # PA1-10011) in Immunocytochemistry

Wed Mar 02 00:00:00 UTC 2022

Acute Exposure to the Food-Borne Pathogen Listeria monocytogenes Does Not Induce α-Synuclein Pathology in the Colonic ENS of Nonhuman Primates.

Journal of inflammation research

Mancinelli AM,Vichich JM,Zinnen AD,Hugon AM,Bondarenko V,Metzger JM,Simmons HA,Golos TG,Emborg ME

Published figure using PGP9.5 polyclonal antibody (Product # PA1-10011) in Immunohistochemistry (PFA fixed)

Fri May 10 00:00:00 UTC 2024

Acute Exposure to the Food-Borne Pathogen Listeria monocytogenes Does Not Induce α-Synuclein Pathology in the Colonic ENS of Nonhuman Primates.

Journal of inflammation research

Mancinelli AM,Vichich JM,Zinnen AD,Hugon AM,Bondarenko V,Metzger JM,Simmons HA,Golos TG,Emborg ME

Published figure using PGP9.5 polyclonal antibody (Product # PA1-10011) in Immunohistochemistry (PFA fixed)

Fri May 10 00:00:00 UTC 2024

生物信息学

蛋白别名: epididymis luminal protein 117; epididymis secretory protein Li 53; gracile axonal dystrophy; Neuron cytoplasmic protein 9.5; OTTHUMP00000218137; OTTHUMP00000218139; OTTHUMP00000218140; OTTHUMP00000218141; PGP 9.5; PGP9.5; protein gene product 9.5; ubiquitin C-terminal hydrolase; ubiquitin C-terminal hydrolase-L1; ubiquitin carboxy-terminal hydrolase L1; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); Ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin carboxyl-terminal hydrolase L1; Ubiquitin thioesterase L1; ubiquitin thiolesterase; ubiquitin thiolesterase L1; UCH-L1; UCHL

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基因别名: AW822034; BOS_6576; C88048; gad; HEL-117; HEL-S-53; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; R75593; UCH-L1; UCHL-1; UCHL1