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PHF8 Antibody (PA5-115751) in ICC/IF
Immunocytochemistry analysis of PHF8 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115751) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
产品信息
PA5-115751
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human PHF8(Accession Q9UPP1), corresponding to amino acid residues Y850-T900.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2900386
产品详细信息
Antibody detects endogenous levels of total PHF8.
靶标信息
PHD finger protein 8 is a jumonji domain containing protein. Like other members of the jumonji family, PHF8 may therefore play a role in histone demethylation. Mutations in PHF8 lead to Siderius type X-linked mental retardation (MRXSSD), a mild to borderline type of mental retardation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: [histone H3]-dimethyl-L-lysine(36) demethylase PHF8; [histone H3]-dimethyl-L-lysine(9) demethylase PHF8; Histone lysine demethylase PHF8; jumonji C domain-containing histone demethylase 1F; PHD finger protein 8
基因别名: 9830141C09Rik; JHDM1F; KIAA1111; mKIAA1111; MRXSSD; PHF8; RGD1561065; ZNF422
UniProt ID: (Human) Q9UPP1, (Mouse) Q80TJ7
Entrez Gene ID: (Human) 23133, (Mouse) 320595, (Rat) 317425
chromatin binding
iron ion binding
protein binding
zinc ion binding
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
histone demethylase activity
histone demethylase activity (H3-K9 specific)
methylated histone binding
histone demethylase activity (H4-K20 specific)
histone demethylase activity (H3-K36 specific)
histone demethylase activity (H3-K27 specific)
molecular_function
G1/S transition of mitotic cell cycle
histone H3-K9 demethylation
histone H4-K20 demethylation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase I promoter
oxidation-reduction process
negative regulation of chromatin silencing at rDNA
histone H3-K36 demethylation
histone H3-K27 demethylation
biological_process
transcription, DNA-templated
brain development
