Invitrogen

PHGDH Monoclonal Antibody (OTI5C4)

PHGDH Antibody in Immunocytochemistry (ICC/IF)

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PHGDH Antibody (MA5-26812) in ICC/IF

Immunofluorescence analysis of PHGDH was performed using 70% confluent log phase NTERA-2 cl.D1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PHGDH Monoclonal Antibody (OTI5C4) (Product # MA5-26812) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A... View More

PHGDH Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PHGDH Antibody — Antibody specificity was demonstrated by the detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of D-3-phosphoglycerate dehydrogenase was observed across the panel tested as compared to A549, MDA-MB-231, and SK-O-V3 which showed low to negative expression as reported in the literature using Anti-PHGDH Monoclonal Antibody (OTI5C4) (Product # MA5-26812) in Western Blot. {RE}

产品信息

MA5-26812

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

免疫组化（石蜡） (IHC (P))

1:150

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

OTI5C4

抗原

Full length human recombinant protein of PHGDH produced in HEK293T cell

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 1% BSA, 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2725014

靶标信息

PHGDH gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 2-oxoglutarate reductase; 3-PGDH; 3-phosphoglycerate dehydrogenase; 3-phosphoglycerate dehyrogenase; A10; D-3-phosphoglycerate dehydrogenase; epididymis secretory protein Li 113; Malate dehydrogenase

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基因别名: 3-PGDH; 3PGDH; 4930479N23; A10; HEL-S-113; NLS; NLS1; PDG; PGAD; PGD; PGDH; PGDH3; PHGDH; PHGDHD; SERA

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UniProt ID: (Human) O43175, (Mouse) Q61753, (Rat) O08651

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Entrez Gene ID: (Human) 26227, (Mouse) 236539, (Rat) 58835

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功能

phosphoglycerate dehydrogenase activity electron carrier activity NAD binding oxidoreductase activity oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor dehydrogenase

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