Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 8
PHIP Antibody (PA5-77167) in ICC/IF
Immunofluorescence analysis of PH-interacting protein (PHIP) was performed using 70% confluent log phase A-431 and U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PHIP Polyclonal Antibody (Product # PA5-77167) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plu... View More
产品信息
PA5-77167
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant full length Human PHIP.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.2, with 50% glycerol
内含物
0.02% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2720894
产品详细信息
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
靶标信息
PHIP (pleckstrin homology domain interacting protein), also known as ndrp or WDR11, is a 1,821 amino acid protein that contains eight N-terminal WD40 repeats and two bromodomains. It is expressed in skeletal muscle (localizing to the cytosol and nucleus) and primary beta cells (localizing to the nucleus) and acts as a transcriptional activator. PHIP is known to interact with various members of the insulin receptor substrate (IRS) family. The IRS family of proteins mediate insulin receptor signaling and play an important role in insulin-producing beta cell proliferation and survival. PHIP specifically associates with the PH domain of IRS-1 and may function to link IRS-1 to insulin receptors, indicating a vital role of PHIP in the regulation of insulin signaling.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: DDB1 and CUL4 associated factor 14; DDB1- and CUL4-associated factor 14; FLJ20705; IRS-1 PH domain-binding protein; neuronal differentiation related protein; neuronal differentiation-related protein; PH-interacting protein; PHIP; WD repeat domain 11; WD repeat-containing protein 11
基因别名: 2810004D21Rik; 4632404O06Rik; BRWD2; DCAF14; Ndrp; PHIP; WDR11
UniProt ID: (Human) Q8WWQ0
Entrez Gene ID: (Human) 55023, (Mouse) 83946
regulation of protein phosphorylation
regulation of transcription from RNA polymerase II promoter
protein import into nucleus
cytoskeleton organization
positive regulation of cell proliferation
insulin receptor signaling pathway
regulation of cell shape
positive regulation of peptidyl-threonine phosphorylation
regulation of cell morphogenesis
positive regulation of peptidyl-serine phosphorylation
regulation of growth
negative regulation of apoptotic process
positive regulation of insulin-like growth factor receptor signaling pathway
positive regulation of mitotic nuclear division
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of extrinsic apoptotic signaling pathway
