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PHKG2 Antibody (PA5-89590) in WB
Western blot analysis of PHKG2 in extracts of various cell lines. Samples were incubated with PHKG2 Polyclonal antibody (Product # PA5-89590) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
产品信息
PA5-89590
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 237-406 of human PHKG2 (NP_0002851)
偶联物
形式
Liquid
浓度
1.11 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2805673
产品详细信息
Immunogen sequence: RQILMLRMIM EGQYQFSSPE WDDRSSTVKD LISRLLQVDP EARLTAEQAL QHPFFERCEG SQPWNLTPRQ RFRVAVWTVL AAGRVALSTH RVRPLTKNAL LRDPYALRSV RHLIDNCAFR LYGHWVKKGE QQNRAALFQH RPPGPFPIMG PEEEGDSAAI TEDEAVLVLG; Positive Samples: MCF-7, 22Rv1, HepG2, Jurkat, 293T, mouse liver, mouse testis, mouse brain, rat liver
靶标信息
PHKG2 is a testis/liver isoform of the phosphorylase kinase gamma subunit. Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by PHKG2. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in PHKG2 cause glycogen storage disease type 9C, also known as autosomal liver glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in PHKG2.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: PHK-gamma-LT; PHK-gamma-T; Phosphorylase b kinase gamma catalytic chain, liver/testis isoform; phosphorylase b kinase gamma catalytic chain, testis/liver isoform; phosphorylase kinase; phosphorylase kinase gamma subunit 2; phosphorylase kinase subunit gamma 2; Phosphorylase kinase subunit gamma-2; phosphorylase kinase, gamma 2 (testis); Phosphorylase kinase, gamma 2 (testis/liver); PSK-C3; serine/threonine-protein kinase PHKG2
基因别名: 1500017I02Rik; GSD9C; PHKG2
UniProt ID: (Human) P15735, (Rat) P31325, (Mouse) Q9DB30
Entrez Gene ID: (Human) 5261, (Rat) 140671, (Mouse) 68961
protein serine/threonine kinase activity
phosphorylase kinase activity
calmodulin binding
ATP binding
enzyme binding
tau-protein kinase activity
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
