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PHPT1 Antibody (MA5-29480) in ICC/IF
Immunofluorescence staining of Human PHPT1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with PHPT1 Recombinant Rabbit Monoclonal Antibody (1) (Product # MA5-29480, 1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
产品信息
MA5-29480
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
001
抗原
Recombinant Human PHPT1 protein (Ala2-Tyr125)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2785338
产品详细信息
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human PHPT1.
靶标信息
The protein encoded by this gene is a member of the protein tyrosine phosphatase family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This protein contains a C-terminal PTP domain and an N-terminal domain homologous to the band 4.1 superfamily of cytoskeletal-associated proteins. P97, a cell cycle regulator involved in a variety of membrane related functions, has been shown to be a substrate of this PTP. This PTP was also found to interact with, and be regulated by adaptor protein 14-3-3 beta. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 14 kDa phosphohistidine phosphatase; epididymis secretory sperm binding protein Li 132P; Phosphohistidine phosphatase 1; PHP; PHPT1; Protein histidine phosphatase; Protein janus-A homolog; PTP-H1; PTPH1; sex-regulated protein janus-a
基因别名: CGI-202; HEL-S-132P; HSPC141; PHP14; PHPT1
UniProt ID: (Human) Q9NRX4
Entrez Gene ID: (Human) 29085
protein dephosphorylation
positive regulation of insulin secretion involved in cellular response to glucose stimulus
peptidyl-histidine dephosphorylation
negative regulation of T cell receptor signaling pathway
negative regulation of lyase activity
positive regulation of cell motility
regulation of actin cytoskeleton reorganization
negative regulation of ATP citrate synthase activity
