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PI3K p55 gamma Antibody (PA5-100897) in ICC/IF
Immunocytochemistry analysis of PI3K p55 gamma in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with PI3K p55 gamma polyclonal antibody (Product # PA5-100897) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjuga... View More
Product Details
PA5-100897
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PIK3R3, corresponding to a region within C-terminal amino acids.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2850392
Product Specific Information
Antibody detects endogenous levels of total PIK3R3.
Target Information
PIK3R3 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1. It is a component of a heterodimer of p110 (catalytic) and p55 (regulatory) subunits. The protein is expressed at highest levels in brain and testis. Lower levels are detected in adipose tissue, kidney, heart, lung and skeletal muscle. The protein contains 2 SH2 domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: p55PIK; phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 (p55); Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; phosphatidylinositol 3-kinase p55 subunit; Phosphatidylinositol 3-kinase regulatory subunit gamma; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 3 (p55, gamma); phosphoinositide-3-kinase, regulatory subunit 3 (gamma); PI3-kinase p85 subunit gamma; PI3-kinase regulatory subunit gamma; PI3-kinase subunit p55-gamma; PI3K regulatory subunit gamma; ptdIns-3-kinase p85-gamma; ptdIns-3-kinase regulatory subunit gamma; ptdIns-3-kinase regulatory subunit p55-gamma; RP4-533D7.2
Gene Aliases: AA414954; p55; p55-GAMMA; p55PIK; PIK3R3
UniProt ID: (Human) Q92569, (Rat) Q63789, (Mouse) Q64143
Entrez Gene ID: (Human) 8503, (Rat) 60664, (Mouse) 18710
phosphatidylinositol biosynthetic process
insulin receptor signaling pathway
phosphatidylinositol-3-phosphate biosynthetic process
regulation of phosphatidylinositol 3-kinase activity
positive regulation of glucose import in response to insulin stimulus
phosphorylation
phosphatidylinositol phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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