Invitrogen

PIAS1 Polyclonal Antibody

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PIAS1 Antibody (PA5-87612) in WB

Western blot analysis of PIAS1 in extracts of various cell lines. Samples were incubated with PIAS1 Polyclonal antibody (Product # PA5-87612) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

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产品信息

PA5-87612

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human PIAS1 (NP_0572501)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.27 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.09% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2804288

产品详细信息

Immunogen sequence: MADSAELKQM VMSLRVSELQ VLLGYAGRNK HGRKHELLTK ALHLLKAGCS PAVQMKIKEL YRRRFPQKIM TPADLSIPNV HSSPMPATLS PSTIPQLTYD GHPASSPLLP VSLLGPKHEL ELPHLTSALH PVHPDIKLQK

Positive Samples: HeLa, A-549, Jurkat, HepG2, Mouse kidney; Cellular Location: Nucleus, Nucleus speckle, PML body

靶标信息

PIAS1 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role in transcriptional coregulation of various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in androgen receptor initiation and maintenance of spermatogenesis. The effects of transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: AR interacting protein; DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1; DEAD/H box-binding protein 1; E3 SUMO-protein ligase PIAS1; E3 SUMO-protein transferase PIAS1; GBP; Gu binding protein; Gu-binding protein; Protein inhibitor of activated STAT protein 1; protein inhibitor of activated STAT-1; RING-type E3 ubiquitin transferase PIAS1; RNA helicase II-binding protein; zinc finger, MIZ-type containing 3

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基因别名: 2900068C24Rik; DDXBP1; GBP; GU/RH-II; PIAS1; ZMIZ3

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UniProt ID: (Human) O75925, (Mouse) O88907

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Entrez Gene ID: (Human) 8554, (Mouse) 56469, (Rat) 300772

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