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PICK1 Antibody (APZ-014-200UL) in IHC (F)
Expression of PICK1 in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-PICK1 Antibody (#APZ-014), (1:400), followed by Anti-rabbit-Cy3 Antibody . PICK1 staining (red in the hippocampal CA3 region) is detected in neurons (arrows). Nuclei are stained with DAPI (blue).
产品信息
APZ-014-200UL
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
(C)RARFSQMRKDVLEK, corresponding to amino acid residues 305-318 of rat PICK1, Intracellular, alpha-helix domain
偶联物
形式
Lyophilized
浓度
0.8 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4, with 1% BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
靶标信息
PSD-95/DLG/ZO-1 (PDZ) domain-containing proteins play a central role in synaptic membrane protein localization. The protein interacting with protein kinase C (PICK1) is a synaptic PDZ domain protein that also contains a coiled-coil and acidic domain. Studies indicate that PICK1 functions as a targeting and transport protein and has been shown to interact with protein kinase C alpha (PKCalpha), AMPA-type glutamate receptors, and several other membrane receptors via its PDZ domain. The interaction of PICK1 with PKCalpha is highly dependent on the activation of the kinase. It appears that PICK1 directs the activated form of PKCalpha to membrane anchored GluR2. Once phosphorylated by PKCalpha, GluR2 is released from the synaptic anchor proteins. Once released, GluR2 is transported from the synaptic membrane in a PICK1-dependent manner.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: MGC15204; OTTHUMP00000028509; PRKCA-binding protein; Protein interacting with C kinase 1; protein kinase C, alpha binding protein; Protein kinase C-alpha-binding protein; protein that interacts with C kinase 1
基因别名: PICK; PICK1; PRKCABP
UniProt ID: (Human) Q9NRD5, (Rat) Q9EP80
Entrez Gene ID: (Human) 9463, (Mouse) 18693, (Rat) 84591
G-protein coupled receptor binding
protein kinase C binding
receptor binding
protein binding
protein C-terminus binding
ATPase activity
enzyme binding
protein domain specific binding
identical protein binding
metal ion binding
actin filament binding
Arp2/3 complex binding
actin binding
kinase activity
receptor tyrosine kinase binding
G-protein coupled glutamate receptor binding
ephrin receptor binding
GTPase binding
vesicle coat protein
positive regulation of receptor internalization
protein phosphorylation
retrograde vesicle-mediated transport, Golgi to ER
protein kinase C-activating G-protein coupled receptor signaling pathway
monoamine transport
glial cell development
regulation of Arp2/3 complex-mediated actin nucleation
negative regulation of Arp2/3 complex-mediated actin nucleation
cellular response to decreased oxygen levels
cellular response to glucose starvation
DNA methylation involved in embryo development
DNA methylation involved in gamete generation
receptor clustering
neuronal ion channel clustering
long term synaptic depression
dendritic spine organization
dendritic spine maintenance
protein targeting
multicellular organism development
negative regulation of gene expression
intracellular signal transduction
regulation of synaptic plasticity
regulation of synapse structure or activity
protein homooligomerization
negative regulation of cell death
dopamine transport
phosphorylation
