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PIGZ Antibody (PA5-89818) in WB
Western blot analysis of PIGZ in extracts of U-87MG cells. Samples were incubated with PIGZ Polyclonal antibody (Product # PA5-89818) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
产品信息
PA5-89818
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 435-579 of human PIGZ (NP_0794392)
偶联物
形式
Liquid
浓度
0.35 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2805790
产品详细信息
Immunogen sequence: LVPGLEYLEQ VVHAPVLPST PTHYTLLFTH TYMPPRHLLH LPGLGAPVEV VDMGGTEDWA LCQTLKSFTR QPACQVAGGP WLCRLFVVTP GTTRRAVEKC SFPFKNETLL FPHLTLEDPP ALSSLLSGAW RDHLSLHIVE LGEET; Positive Samples: U-87MG; Cellular Location: Endoplasmic reticulum membrane, Multi-pass membrane protein
靶标信息
The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells that serves to anchor proteins to the cell surface. This gene encodes a protein that is localized to the endoplasmic reticulum, and is involved in GPI anchor biosynthesis. As shown for the yeast homolog, which is a member of a family of dolichol-phosphate-mannose (Dol-P-Man)-dependent mannosyltransferases, this protein can also add a side-branching fourth mannose to GPI precursors during the assembly of GPI anchors.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: dol-P-Man dependent GPI mannosyltransferase; GPI mannosyltransferase 4; GPI mannosyltransferase IV; GPI-MT-IV; hSMP3; phosphatidylinositol glycan anchor biosynthesis, class Z; phosphatidylinositol glycan, class Z; Phosphatidylinositol-glycan biosynthesis class Z protein; PIG-Z; SMP3 homolog; SMP3 mannosyltransferase
基因别名: GPI-MT-IV; PIG-Z; PIGZ; SMP3
UniProt ID: (Human) Q86VD9
Entrez Gene ID: (Human) 80235
