Invitrogen

PIK3CB Recombinant Rabbit Monoclonal Antibody (SC0580)

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PIK3CB Antibody (MA5-32208) in WB

Western blot was performed using Anti-PIK3CB Recombinant Rabbit Monoclonal Antibody (SC0580) (Product # MA5-32208) and a 110kDa band corresponding to Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform was observed across the cell lines tested and the expression was found to be low in MDA-MB-231 when compared to T-47D. Nuclear enriched extracts (30 µg lysate) of MCF7 (Lane 1), DU 145 (Lane 2), T-47D (Lane 3), MDA-MB-231 (Lane 4), NIH:OVCAR-3 (Lane 5), HEL 92.1.7 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bi... View More

PIK3CB Antibody in Flow Cytometry (Flow)

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产品信息

MA5-32208

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

流式细胞分析 (Flow)

1:50-1:100

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

SC0580

抗原

Synthetic peptide within Human PIK3CB aa 5-52

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2809495

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

PIK3CB (phosphatyidylinositol 4,5-biphosphate 3-kinase catalyic subunit beta isoform) is a phosphoinositide-3-kinase that phosphorylates PtdIns (phosphatidylinositol), PtdIns4P (phosphatidylinositol 4-phosphate), and PtdIns(4,5)P2 (phosphatidylinositol 4,5-biphosphate) to generate phosphatidylinositol 3,4,5-triphosphate (PIP3). PIP3 plays a key role in recruiting PH domain-containing proteins to the membrane and activating signaling cascades involved in cell growth, survival, proliferation, motility, and morphology.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta; Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta; phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit beta; phosphoinositide-3-kinase, catalytic, beta polypeptide; PI3-kinase p110 subunit beta; PI3-kinase subunit beta; PI3K-beta; pi3kcb; PtdIns-3-kinase p110; ptdIns-3-kinase subunit beta; PtdIns-3-kinase subunit p110-beta; Serine/threonine protein kinase PIK3CB

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基因别名: P110BETA; PI3K; PI3KBETA; PIK3C1; PIK3CB

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UniProt ID: (Human) P42338

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Entrez Gene ID: (Human) 5291

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功能

protein binding ATP binding 1-phosphatidylinositol-3-kinase activity phosphatidylinositol 3-kinase activity 1-phosphatidylinositol-4-phosphate 3-kinase activity insulin receptor substrate binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity kinase

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