Invitrogen

PINK1 Polyclonal Antibody

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抗体检测数据 (4)

PINK1 Antibody in Immunocytochemistry (ICC/IF)

PINK1 Antibody in Immunocytochemistry (ICC/IF)

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PINK1 Antibody (PA5-85243) in ICC/IF

PINK1 Polyclonal Antibody detects PINK1 protein at mitochondria by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: PINK1 protein stained by PINK1 Polyclonal Antibody (Product # PA5-85243) diluted at 1:500. Blue: Hoechst 33342 staining.

PINK1 Antibody in Immunocytochemistry (ICC/IF)

PINK1 Antibody in Immunocytochemistry (ICC/IF)

PINK1 Antibody in Western Blot (WB)

PINK1 Antibody in Western Blot (WB)

Product Image Gallery

PINK1 Antibody in Immunocytochemistry (ICC/IF) — PINK1 Polyclonal Antibody detects PINK1 protein at mitochondria by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: PINK1 protein stained by PINK1 Polyclonal Antibody (Product # PA5-85243) diluted at 1:500. Blue: Hoechst 33342 staining.

PINK1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of PINK1 in MCF-7 cells using PINK1 polyclonal antibody (Product # PA5-85243) at a dilution of 1:500. Sample was then incubated with Hoechst secondary antibody.

PINK1 Antibody in Western Blot (WB) — Western blot analysis of PINK1 in A) A431 cells using PINK1 polyclonal antibody (Product # PA5-85243) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS PAGE.

PINK1 Antibody in Western Blot (WB) — Western Blot using PINK1 Polyclonal Antibody (Product # PA5-85243). Sample (30 µg whole cell lysate). A: A431. 7.5% SDS PAGE. PINK1 Polyclonal Antibody (Product # PA5-85243) diluted at 1:1,000.

产品信息

PA5-85243

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

-

免疫细胞化学 (ICC/IF)

1:100-1:1,000

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human PINK1.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 10% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2792386

产品详细信息

Keep as concentrated solution.

Predicted reactivity: Mouse (84%), Bovine (88%).

Positive Control: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

PINK1 (PTEN-induced putative kinase 1) protects from mitochondrial dysfunction in cells by conferring different autophosphorylation activity to mitochondrial genes. The kinase domain of PINK1 appears to be critical in its protective effects. Mitochondrial proteins are critical for the regulation of cellular energy and adaptation to stress. Particularly in brain cells, mitochondrial demand is extraordinarily high, in order to accommodate aerobic and anaerobic support of high energy processes. When mitochondrial structure and/or function is disrupted in the brain, neurodegenerative diseases like Parkinson's disease are triggered.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: BRPK; FLJ27236; PINK 1; PINK-1; protein kinase BRPK; PTEN-induced putative kinase protein 1; Serine/threonine-protein kinase PINK1, mitochondrial

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基因别名: 1190006F07Rik; AU042772; AW557854; BRPK; mFLJ00387; PARK6; PINK1

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UniProt ID: (Human) Q9BXM7, (Mouse) Q99MQ3

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Entrez Gene ID: (Human) 65018, (Mouse) 68943

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magnesium ion binding protease binding protein kinase activity protein serine/threonine kinase activity protein binding ATP binding calcium-dependent protein kinase activity kinase activity peptidase activator activity ubiquitin protein ligase binding protein kinase B binding C3HC4-type RING finger domain binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups metal ion binding non-receptor serine/threonine protein kinase protein modifying enzyme

mitophagy positive regulation of protein phosphorylation regulation of oxidative phosphorylation protein phosphorylation ubiquitin-dependent protein catabolic process response to stress response to oxidative stress mitochondrion organization regulation of hydrogen peroxide metabolic process negative regulation of gene expression regulation of mitochondrion organization positive regulation of peptidase activity macroautophagy positive regulation of macroautophagy negative regulation of macroautophagy phosphorylation protein ubiquitination peptidyl-serine phosphorylation respiratory electron transport chain regulation of protein ubiquitination positive regulation of protein ubiquitination activation of protein kinase B activity positive regulation of synaptic transmission, dopaminergic positive regulation of peptidyl-serine phosphorylation positive regulation of dopamine secretion cellular response to oxidative stress positive regulation of protein dephosphorylation intracellular signal transduction peptidyl-serine autophosphorylation TORC2 signaling positive regulation of I-kappaB kinase/NF-kappaB signaling regulation of protein complex assembly negative regulation of neuron apoptotic process positive regulation of translation negative regulation of JNK cascade protein stabilization positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of ubiquitin-protein transferase activity regulation of mitochondrial membrane potential positive regulation of protein kinase B signaling regulation of proteasomal protein catabolic process cellular response to hypoxia establishment of protein localization to mitochondrion maintenance of protein location in mitochondrion positive regulation of mitochondrial fission positive regulation of release of cytochrome c from mitochondria negative regulation of mitochondrial fission cellular response to toxic substance mitophagy in response to mitochondrial depolarization regulation of synaptic vesicle transport negative regulation of autophagosome assembly positive regulation of mitochondrial electron transport, NADH to ubiquinone regulation of mitophagy negative regulation of mitophagy negative regulation of oxidative stress-induced cell death negative regulation of oxidative stress-induced neuron death regulation of protein targeting to mitochondrion negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway negative regulation of hydrogen peroxide-induced neuron intrinsic apoptotic signaling pathway negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide positive regulation of cristae formation positive regulation of protein targeting to mitochondrion positive regulation of free ubiquitin chain polymerization regulation of reactive oxygen species metabolic process negative regulation of reactive oxygen species metabolic process positive regulation of ATP biosynthetic process autophagy positive regulation of catecholamine secretion regulation of neuron apoptotic process negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway