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PIWIL2 Antibody (PA5-110174) in WB
Western blot analysis of PIWIL2 in extracts of various cell lines. Samples were incubated with PIWIL2 Polyclonal antibody (Product # PA5-110174) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
PA5-110174
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 398-652 of mouse PIWIL2 (Q8CDG1)
Conjugate
Form
Liquid
Concentration
3.18 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2855585
Product Specific Information
Immunogen sequence: QNKEHFQDEC SKLLVGSIVI TRYNNRTYRI DDVDWNKTPK DSFVMSDGKE ITFLEYYSKN YGITVKEDDQ PLLIHRPSER QNNHGMLLKG EILLLPELSF MTGIPEKMKK DFRAMKDLTQ QINLSPKQHH GALECLLQRI SQNETASNEL TRWGLSLHKD VHKIEGRLLP MERINLRNTS FVTSEDLNWV KEVTRDASIL TIPMHFWALF YPKRAMDQAR ELVNMLEKIA GPIGMRISPP AWVELKDDRI ETYIR
Target Information
The piwi gene of Drosophila belongs to a novel class of evolutionarily conserved genes (the piwi or Argonaute family). The Piwi family is required for germ and stem cell development in invertebrates, and two Piwi members, MIWI and MILI (Miwi like, Piwil2, Piwi-like protein 2), are essential for spermatogenesis in mouse. MIWI and MILI partner with Piwi-interacting RNAs (piRNAs) which are 26-31 nucleotides in length clearly distinct from the 21-23 nucleotides of microRNAs (miRNAs) or short interfering RNAs (siRNAs). A search for murine small RNAs that might program Piwi proteins for transposon suppression revealed developmentally regulated piRNA loci, some of which resemble transposon master control loci of Drosophila. MILI might be programmed by distinct piRNA populations at different stages of germ cell development, suggesting MILI plays an important role in meiotic prophase progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Miwi like; Miwi likw; piwi like homolog 2; PIWI-L2; piwi-like 2; piwi-like homolog 2; Piwi-like protein 2
Gene Aliases: Mili; Piwil1l; Piwil2
UniProt ID: (Mouse) Q8CDG1
Entrez Gene ID: (Rat) 306011, (Mouse) 57746
RNA 5'-end processing
spermatogenesis
germ-line stem cell population maintenance
gene silencing by RNA
piRNA metabolic process
DNA methylation involved in gamete generation
positive regulation of translation
oogenesis
positive regulation of meiosis I
pachytene
regulation of translation
multicellular organism development
cell differentiation
meiotic cell cycle
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