Invitrogen

PKA C alpha/beta Polyclonal Antibody

PKA C alpha/beta Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 5

PKA C alpha/beta Antibody (PA5-115763) in ICC/IF

Immunocytochemistry analysis of PKA C alpha/beta in HeLa. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115763) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with Alexa Fluor 594 conjugated goat anti-rabbit IgG using a dilution of 1:600.

PKA C alpha/beta Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PKA C alpha/beta Antibody in Western Blot (WB)

Product Image Gallery

产品信息

PA5-115763

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human PRKACA(Accession P17612), corresponding to amino acid residues M1-I47.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2900398

产品详细信息

Antibody detects endogenous levels of total PKA alpha/beta CAT.

靶标信息

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. PRKACB is a member of the Ser/Thr protein kinase family and is a catalytic subunit of AMPK.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: cAMP-dependent protein kinase C beta; cAMP-dependent protein kinase catalytic beta subunit isoform 4ab; cAMP-dependent protein kinase catalytic subunit alpha; cAMP-dependent protein kinase catalytic subunit alpha, isoform 1; cAMP-dependent protein kinase catalytic subunit beta; cAMP-dependent protein kinase catalytic subunit C alpha; PKA C-alpha; PKA C-beta; protein kinase A; protein kinase A catalytic subunit; protein kinase A catalytic subunit beta; protein kinase, cAMP-dependent, alpha catalytic subunit; protein kinase, cAMP-dependent, beta catalytic subunit; protein kinase, cAMP-dependent, catalytic, alpha; protein kinase, cAMP-dependent, catalytic, beta; sperm cAMP-dependent protein kinase catalytic subunit

查看更多收起

基因别名: Cs-PKA; PKA C-beta; PKACA; PKACB; PKCA1; PKCD; PPNAD4; PRKACA; PRKACB