Invitrogen

PKC alpha Monoclonal Antibody (MC5)

PKC alpha Antibody in Immunocytochemistry (ICC/IF)

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PKC alpha Antibody (MA1-157) in ICC/IF

Immunofluorescent analysis of PKC alpha (green) in SH-SY5Y and MCF7 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a PKC alpha monoclonal antibody (Product # MA1-157) at a dilution of 1:100 for at least 1 hour at room temperatur... View More

PKC alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PKC alpha Antibody in Immunoprecipitation (IP)

PKC alpha Antibody in Immunohistochemistry (IHC)

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产品信息

MA1-157

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

查看5篇已发表文章 5篇已发表文章

免疫组化（石蜡） (IHC (P))

1:10-1:100

免疫细胞化学 (ICC/IF)

1:50-1:100

查看3篇已发表文章 3篇已发表文章

免疫沉淀 (IP)

5 µg

产品规格

种属反应

Bovine, Dog, Human, Mouse, Non-human primate, Rabbit, Rat

已发表种属

Human, Mouse, Rabbit, Rat

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

MC5

抗原

Purified bovine brain Protein Kinase C alpha.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with 30% glycerol, 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2536865

产品详细信息

Western blot analysis of MA1-157 detects an ~80 kDa protein in human, bovine, non-human primate, mouse, rat, dog and rabbit cells. In Jurkat, rat brain and MDCK cell lysates, a weak unknown band at ~140 kDa is also detected. MA1-157 shows specificity to PKC alpha and is non-reactive to other PKC family members.

靶标信息

The PKC family of serine/threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Nicorandil mitigates amiodarone-induced pulmonary toxicity and fibrosis in association with the inhibition of lung TGF-β1/PI3K/Akt1-p/mTOR axis in rats.

Clinical and experimental pharmacology & physiology

Harb I,Ashour H,Rashed LA,Mostafa A,Samir M,Aboulhoda BE,El-Hanbuli H,Rashwan E,Mahmoud H

MA1-157 was used in Western Blotting to suggest NI administration as a preventive therapy from the serious pulmonary fibrosis side effect of AMIO.

Sun Jan 01 00:00:00 UTC 2023

Photoreceptor laminin drives differentiation of human pluripotent stem cells to photoreceptor progenitors that partially restore retina function.

Molecular therapy : the journal of the American Society of Gene Therapy

Tay HG,Andre H,Chrysostomou V,Adusumalli S,Guo J,Ren X,Tan WS,Tor JE,Moreno-Moral A,Plastino F,Bartuma H,Cai Z,Tun SBB,Barathi VA,Siew Wei GT,Grenci G,Chong LY,Holmgren A,Kvanta A,Guy CJ,Petretto E,Tryggvason K

MA1-157 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to investigate the role of a human recombinant retina-specific laminin isoform, LN523 in promoting the differentiation of human embryonic stem cells into photoreceptor progenitors.

Wed Mar 01 00:00:00 UTC 2023

Prion-induced photoreceptor degeneration begins with misfolded prion protein accumulation in cones at two distinct sites: cilia and ribbon synapses.

Acta neuropathologica communications

Striebel JF,Race B,Leung JM,Schwartz C,Chesebro B

MA1-157 was used in Immunocytochemistry to study deposition of disease-associated prion protein (PrPSc) and its association with damage to critical retinal structures following intracerebral prion inoculation.

Fri Jan 29 00:00:00 UTC 2021

Prion-induced photoreceptor degeneration begins with misfolded prion protein accumulation in cones at two distinct sites: cilia and ribbon synapses.

Acta neuropathologica communications

Striebel JF,Race B,Leung JM,Schwartz C,Chesebro B

Fri Jan 29 00:00:00 UTC 2021

Prion-induced photoreceptor degeneration begins with misfolded prion protein accumulation in cones at two distinct sites: cilia and ribbon synapses.

Acta neuropathologica communications

Striebel JF,Race B,Leung JM,Schwartz C,Chesebro B

Fri Jan 29 00:00:00 UTC 2021

Large-Area Photoreceptor Degeneration Model in Rabbits by Photocoagulation and Oxidative Stress in the Retina.

Frontiers in neuroscience

Wang Z,Feng C,Yang R,Liu T,Chen Y,Chen A,Yan B,Yuan Y,Zhang J

MA1-157 was used in Immunohistochemistry to examine different parameters of photocoagulation on lesions of the retina in a rabbit.

Thu Aug 12 00:00:00 UTC 2021

Large-Area Photoreceptor Degeneration Model in Rabbits by Photocoagulation and Oxidative Stress in the Retina.

Frontiers in neuroscience

Wang Z,Feng C,Yang R,Liu T,Chen Y,Chen A,Yan B,Yuan Y,Zhang J

MA1-157 was used in Immunohistochemistry to examine different parameters of photocoagulation on lesions of the retina in a rabbit.

Thu Aug 12 00:00:00 UTC 2021

A microfluidic platform enables comprehensive gene expression profiling of mouse retinal stem cells.

Lab on a chip

Coles BLK,Labib M,Poudineh M,Innes BT,Belair-Hickey J,Gomis S,Wang Z,Bader GD,Sargent EH,Kelley SO,van der Kooy D

MA1-157 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to identify transcription factors (TFs) that play functional roles in maintenance, quiescence, and proliferation of RSCs.

Tue Nov 09 00:00:00 UTC 2021

A dual role for Ca_v1.4 Ca²⁺ channels in the molecular and structural organization of the rod photoreceptor synapse.

eLife

Maddox JW,Randall KL,Yadav RP,Williams B,Hagen J,Derr PJ,Kerov V,Della Santina L,Baker SA,Artemyev N,Hoon M,Lee A

MA1-157 was used in Immunohistochemistry to support a model in which presynaptic Cav channels serve both as organizers of synaptic building blocks and as sources of Ca2+ ions in building the first synapse of the visual pathway and perhaps more broadly in the nervous system.

Thu Sep 17 00:00:00 UTC 2020

Tmem30a deficiency leads to retinal rod bipolar cell degeneration.

Journal of neurochemistry

Yang Y,Liu W,Sun K,Jiang L,Zhu X

Published figure using PKC alpha monoclonal antibody (Product # MA1-157) in Immunofluorescence

Fri Feb 01 00:00:00 UTC 2019

Localization of Rod Bipolar Cells in the Mammalian Retina Using an Antibody Against the α1c L-type Ca(2+) Channel.

Acta histochemica et cytochemica

Huh YJ,Choi JS,Jeon CJ

MA1-157 was used in immunohistochemistry to test if the alpha1c subunit of L-type voltage-gated calcium channel colocalizes with protein kinase C alpha in the rod bipolar cells in mouse, hamster, rabbit, and dog retinas

Tue Apr 28 00:00:00 UTC 2015

生物信息学

蛋白别名: aging-associated gene 6; EC 2.7.11.13; FHC; FTH; FTHL6; kinase PKC-alpha; KPCA; MGC104426; PIG15; PKC III; PKC-A; PKC-alpha; PKC-III; PLIF; protein kinase C; Protein kinase C alpha type; protein kinase C, alpha; protein kinase c-alpha; unnamed protein product

查看更多收起

基因别名: AAG6; AI875142; PKC-alpha; PKCA; PKRCA; PRKACA; PRKCA

查看更多收起

功能

protein kinase C activity calcium-dependent protein kinase C activity protein binding ATP binding zinc ion binding PDZ domain binding scaffold protein binding protein kinase activity protein serine/threonine kinase activity enzyme binding histone kinase activity (H3-T6 specific) nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups metal ion binding non-receptor serine/threonine protein kinase

参与通路

PKC alpha Monoclonal Antibody (MC5)

PKC alpha Antibody (MA1-157) in ICC/IF

产品信息

MA1-157

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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