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PKC eta Antibody (PA5-79871) in WB
Western blot analysis of PKC eta in Lane 1: A431 whole cell lysate, Lane 2: A549 whole cell lysate, Lane 3: HeLa whole cell lysate, Lane 4: SKOV whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with PKC eta polyclonal antibody (Product # PA5-79871)... View More
Product Details
PA5-79871
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human PKC eta recombinant protein (Position: R30-D389).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746986
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: A431 whole cell, A549 whole cell, HELA whole cell, SKOV whole cell. Flow: A431 cell, K562 cell, MCF-7 cell.
Target Information
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC26269; MGC5363; nPKC-eta; PKC-L; Protein kinase C eta type; protein kinase C, eta
Gene Aliases: nPKC-eta; PKC-L; PKCL; PRKCH; PRKCL
UniProt ID: (Human) P24723
Entrez Gene ID: (Human) 5583
protein phosphorylation
signal transduction
positive regulation of macrophage derived foam cell differentiation
peptidyl-serine phosphorylation
platelet activation
negative regulation of glial cell apoptotic process
intracellular signal transduction
positive regulation of keratinocyte differentiation
positive regulation of B cell receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
positive regulation of glial cell proliferation
protein kinase C signaling
regulation of bicellular tight junction assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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