Invitrogen

PKM Monoclonal Antibody (6C3C7)

PKM Antibody in Immunocytochemistry (ICC/IF)

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PKM Antibody (MA5-38278) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis of PKM1/PKM2 in A549 cells using PKM Monoclonal Antibody (Product # MA5-38278) diluted at 1:230, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

PKM Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PKM Antibody in Immunoprecipitation (IP)

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产品信息

MA5-38278

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:4,000-1:256,000

免疫组化（石蜡） (IHC (P))

1:200-1:500

免疫细胞化学 (ICC/IF)

1:150-1:300

流式细胞分析 (Flow)

1:50-1:200

免疫沉淀 (IP)

1-4 µL

产品规格

种属反应

Human, Mouse, Rabbit, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

6C3C7

抗原

Recombinant Human Pyruvate kinase PKM protein (2-531aa)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

4.66 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.03% ProClin 300

保存条件

-20°C or -80°C if preferred

运输条件

Wet ice

AB_2898193

产品详细信息

This antibody has been tested in direct-ELISA

靶标信息

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: ATP:pyruvate 2-o-phosphotransferase; CTHBP; Cytosolic thyroid hormone-binding protein; epididymis secretory protein Li 30; m2pk; OIP-3; OPA-interacting protein 3; p58; PK, muscle type; Pyruvate kinase 2/3; pyruvate kinase 3; Pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle isozyme; Pyruvate kinase PKM; pyruvate kinase, muscle; THBP1; Threonine-protein kinase PKM2; Thyroid hormone-binding protein 1; thyroid hormone-binding protein, cytosolic; Tumor M2-PK; Tyrosine-protein kinase PKM2

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基因别名: AA414905; AL024370; AL024424; CTHBP; HEL-S-30; OIP3; Pk-2; Pk-3; PK2; PK3; PKM; PKM12; PKM2; Pykm; TCB; THBP1