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PKM2 Antibody (600-401-GV5) in WB
Western Blot of Rabbit Anti-PKM2 antibody. Lane 1 and 2: iPSC lysate. Lane 3 and 4: myeloid lysate (iPSC derived). Load: 100 µg per lane. Primary antibody: PKM2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Odyssey blocking buffer for 120 min at RT. Predicted/Observed size: 57 kDa for PKM2. Other band(s): PKM2 splice variants and isoforms as PKM2 is a highly modified protein.
产品信息
600-401-GV5
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Affinity purified Anti-PKM2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the internal portion of human PKM2 protein.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-PKM2 is directed against human PKM2 at an internal position. A BLAST analysis was used to suggest reactivity with this protein in African frog and rat based on 100% homology and rabbit and mouse based on 93.3% homology for the immunogen sequence.
靶标信息
PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. PKM2 has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. PKM2 has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms of PKM2 have been reported.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CTHBP; Cytosolic thyroid hormone-binding protein; epididymis secretory protein Li 30; isozymes M1/M2; m2pk; OIP-3; OPA-interacting protein 3; p58; PK, muscle type; Pyruvate kinase 2/3; pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle isozyme; Pyruvate kinase PKM; THBP1; Threonine-protein kinase PKM2; Thyroid hormone-binding protein 1; thyroid hormone-binding protein, cytosolic; tu; Tumor M2-PK; Tyrosine-protein kinase PKM2
基因别名: CTHBP; HEL-S-30; OIP3; PK2; PK3; PKM; PKM2; TCB; THBP1
UniProt ID: (Human) P14618
Entrez Gene ID: (Human) 5315
response to hypoxia
liver development
ATP biosynthetic process
response to nutrient
response to gravity
programmed cell death
response to muscle inactivity
organ regeneration
response to insulin
pyruvate biosynthetic process
skeletal muscle tissue regeneration
canonical glycolysis
cell-cell adhesion
