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PKN2 Antibody (MA5-44818) in ICC/IF
Immunocytochemistry analysis of PKN2 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Samples were incubated in PKN2 Monoclonal antibody (Product # MA5-44818) using a dilution of 1:50 for 1 hour at room temperature, washed with PBS followed by Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG secondary antibody at a dilution of 1:1,000. The nuclear counter stain is DAPI (blue).
产品信息
MA5-44818
产品规格
种属反应
Human,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JE62-44
抗原
Recombinant protein within human PKN2 aa 885-984/984.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2931275
靶标信息
PKN2 (PRK2), a close relative of the PKCs, is a regulator of mitosis and exit from cytokinesis. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c-fos serum response factor (SRF). Phosphorylates HCV NS5B leading to stimulation of HCV RNA replication.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: cardiolipin-activated protein kinase Pak2; p140 kinase; PKN gamma; protease-activated kinase 2; Protein kinase C-like 2; Protein-kinase C-related kinase 2; RP5-905H16.1; Serine/threonine-protein kinase N2
基因别名: Pak-2; PAK2; PKN2; PRK2; PRKCL2; PRO2042; STK7
UniProt ID: (Human) Q9H1W4
Entrez Gene ID: (Human) 5586, (Rat) 207122
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
apoptotic process
cell cycle
signal transduction
epithelial cell migration
peptidyl-serine phosphorylation
positive regulation of cytokinesis
intracellular signal transduction
apical junction assembly
positive regulation of viral genome replication
positive regulation of mitotic cell cycle
cell division
cell-cell adhesion
regulation of cell motility
cell adhesion
