Invitrogen

PLA2G2E Polyclonal Antibody

PLA2G2E Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 2

PLA2G2E Antibody (PA5-69443) in IHC (P)

Immunohistochemical analysis of PLA2G2E in paraffin-embedded human placenta tissue. Sample was probed with a PLA2G2E polyclonal antibody (Product # PA5-69443) at a dilution of 1:200. Detection was performed with a donkey anti-Rabbit Cy3 secondary antibody at a dilution of 1:200. Images were taken at 20x magnification with an exposure time of 0.5-2.0 seconds.

Product Image Gallery

产品信息

PA5-69443

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.2-1.0 µg/mL

免疫组化 (IHC)

1:100

免疫组化（石蜡） (IHC (P))

Assay-Dependent

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

synthetic peptide directed towards the middle region of human PLA2G2E

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS with 2% sucrose

内含物

0.09% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2688323

产品详细信息

This target displays homology in the following species: Cow: 100%; Horse: 93%; Human: 100%; Mouse: 100%

靶标信息

Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids (PubMed:10681567, PubMed:11922621, PubMed:28883454). Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity), releasing various unsaturated fatty acids including oleoate, linoleoate, arachidonate, docosahexaenoate and lysophosphatidylethanolamines in preference to lysophosphatidylcholines (PubMed:10681567, PubMed:28883454). In response to high-fat diet, hydrolyzes minor lipoprotein phospholipids including phosphatidylserines, phosphatidylinositols and phosphatidylglycerols, altering lipoprotein composition and fat storage in adipose tissue and liver. May act in an autocrine and paracrine manner (PubMed:11922621). Contributes to lipid remodeling of cellular membranes and generation of lipid mediators involved in pathogen clearance. Cleaves sn-2 fatty acyl chains of phosphatidylglycerols and phosphatidylethanolamines, which are major components of membrane phospholipids in bacteria (PubMed:11922621). Acts as a hair follicle phospholipase A2. Selectively releases lysophosphatidylethanolamines (LPE) and various unsaturated fatty acids in skin to regulate hair follicle homeostasis. May regulate the inflammatory response by releasing arachidonate, a precursor of prostaglandins and leukotrienes (PubMed:11922621). Upon allergen exposure, may participate in allergic inflammatory response by enhancing leukotriene C4 synthesis and degranulation in mast cells. [UniProt]

仅用于科研。不用于诊断过程。未经明确授权不得转售。