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PLCG1 Antibody (PA5-120513) in WB
Western blot analysis of PLCG1 in extracts of various cell lines. Samples were incubated with PLCG1 Polyclonal antibody (Product # PA5-120513) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
PA5-120513
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence within amino acids 700-800 of human PLC gamma 1 (PLCG1) (NP_002651.2).
偶联物
形式
Liquid
浓度
1.32 mg/mL
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2914085
产品详细信息
Positive test controls include: MCF7, Jurkat. The target is usually found in the following locations: Cell projection, lamellipodium, ruffle.
Immunogen sequence: NSYAISFRAE GKIKHCRVQQ EGQTVMLGNS EFDSLVDLIS YYEKHPLYRK MKLRYPINEE ALEKIGTAEP DYGALYEGRN PGFYVEANPM PTFKCAVKAL F
靶标信息
The mammalian Phospholipase Cg (PLCg) family has two closely related proteins, PLCg1 and PLCg2. The PLCg isozymes have the core structure domains and a unique array of domains containing an additional PH domain, two SH2 domains and one SH3 domain. In response to extracellular stimuli, such as hormones and growth factors, receptor tyrosine kinases (RTKs) phosphorylate and activate PLCg. Activated PLCg catalyzes hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to produce the metabolic second messenger molecules inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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