Invitrogen

PLK2 Polyclonal Antibody

PLK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 8

PLK2 Antibody (PA5-14094) in IHC (P)

Immunohistochemistry analysis of PLK2 in formalin-fixed and paraffin-embedded human breast carcinoma tissue. Samples were incubated with PLK2 polyclonal antibody (Product # PA5-14094) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

PLK2 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

PLK2 Antibody — Figure 3. Hyperactivity induces Plk2 which is required for hyperactivity induction of NF-kappaB. A , Representative immunoblot (inset) and quantification of Plk2 levels in lysates from chronic PTX (PTX, 100 mu m , 24 h) or mock-treated DIV21 hippocampal cultures in the presence (Ctrl or PTX) or absence of p65 (OHT or OHT/PTX). HSC70 was used as the loading control (* p <= 4.54 x 10 -4 , ANOVA; n = 5 independent experiments). B , NF-kappaB luciferase reporter in DIV19 hippocampal neurons. NF-kappaB transcriptional activity is significantly increased by 12 h PTX treatment (50-100 mu m ; * p = 0.003), but this is prevented by expression of the kinase-dead Plk2 K108M ( # p = 0.01; n = 3 independent experiments). C , NF-kappaB limits decreases in spine density by chronic PTX. Hippocampal neurons (DIV21, RelA F/F mice) in the presence or absence of p65 (Cre expression 48 h) were vehicle or PTX treated (100 mu m for 24 h; * p <= 1.62 x 10 -4 , ANOVA; n >= 15 dendritic segments from 4 independent experiments). D , NF-kappaB limits decreases in spine volumes by chronic PTX. Spine head volumes were measured using Imaris software from the same dendritic segments as in A (* p <= 5.42 x 10 -4 , ANOVA). E , Confocal projections of representative dendrite segments for data in C and D . Scale bar, 10 mum. All error bars indicate SEM. {TM}

产品信息

PA5-14094

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:100

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 375-406 amino acids from the C-terminal region of human PLK2 (SNK)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Antigen affinity chromatography, Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2167742

靶标信息

PLK2 is a serum inducible serine/threonine kinase that is a member of the polo family of serine/threonine kinases which have a role in normal cell division. The kinase may play a role in embryogenesis, wound healing, and neoplasia.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Targeting Adaptive IRE1α Signaling and PLK2 in Multiple Myeloma: Possible Anti-Tumor Mechanisms of KIRA8 and Nilotinib.

International journal of molecular sciences

Yamashita Y,Morita S,Hosoi H,Kobata H,Kishimoto S,Ishibashi T,Mishima H,Kinoshita A,Backes BJ,Yoshiura KI,Papa FR,Sonoki T,Tamura S

Published figure using PLK2 polyclonal antibody (Product # PA5-14094) in Immunohistochemistry

Mon Aug 31 00:00:00 UTC 2020

Targeting Adaptive IRE1α Signaling and PLK2 in Multiple Myeloma: Possible Anti-Tumor Mechanisms of KIRA8 and Nilotinib.

International journal of molecular sciences

Yamashita Y,Morita S,Hosoi H,Kobata H,Kishimoto S,Ishibashi T,Mishima H,Kinoshita A,Backes BJ,Yoshiura KI,Papa FR,Sonoki T,Tamura S

Published figure using PLK2 polyclonal antibody (Product # PA5-14094) in Immunohistochemistry

Mon Aug 31 00:00:00 UTC 2020

Opposing action of nuclear factor κB and Polo-like kinases determines a homeostatic end point for excitatory synaptic adaptation.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Mihalas AB,Araki Y,Huganir RL,Meffert MK

PA5-14094 was used in western blot to study excitory synaptic homeostatic end point adaptation and the roles played by NFkB and Polo-like kinases

Wed Oct 16 00:00:00 UTC 2013

生物信息学

蛋白别名: h PLK-2; hSNK; PLK-2; polo-like kinase 1; Polo-like kinase 2; Serine/threonine-protein kinase PLK2; Serine/threonine-protein kinase SNK; Serum-inducible kinase

查看更多收起

基因别名: hPlk2; hSNK; PLK2; SNK

查看更多收起

UniProt ID: (Human) Q9NYY3, (Mouse) P53351, (Rat) Q9R012

查看更多收起

Entrez Gene ID: (Human) 10769, (Mouse) 20620, (Rat) 83722

查看更多收起

功能