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PLK4 Antibody (PA5-29373) in WB
Western Blot analysis of PLK4 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PLK4 Polyclonal Antibody (Product # PA5-29373) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
产品信息
PA5-29373
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 614 and 871 of Human PLK4
偶联物
形式
Liquid
浓度
0.19 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol, 1% BSA
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2546849
产品详细信息
Recommended positive controls: PLK4-transfected 293T.
Predicted reactivity: Bovine (87%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
PLK4 or polo-like kinase 4 is a member of the polo family of serine/threonine protein kinases which localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. The overexpression of PLK4 triggered the simultaneous formation of multiple procentrioles around each preexisting centriole which resulting in centriole amplification and thus, Plk4-induced centriole biogenesis in human cells. The reduced Plk4 gene dosage increases the probability of mitotic errors and cancer development.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: PLK-4; Polo-like kinase 4; Serine/threonine-protein kinase 18; Serine/threonine-protein kinase PLK4; Serine/threonine-protein kinase Sak; Snk akin kinase
基因别名: MCCRP2; PLK4; SAK; STK18
UniProt ID: (Human) O00444
Entrez Gene ID: (Human) 10733
